bs-41322R [Primary Antibody]
CH25H
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Host: Rabbit

Target Protein: CH25H

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9023

Swiss Prot: O95992

Source: Recombinant human CH25H protein

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

CH25H is involved in cholesterol and lipid metabolism and catalyses the formation of 25-hydroxycholesterol from cholesterol, leading to repression of cholesterol biosynthetic enzymes. It may play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing.

Size: 100ul

Concentration: 1mg/ml

Applications: WB(WB=1:500-2000)
IHC-P(IHC-P=1:400-800)
IHC-F(IHC-F=1:400-800)
IF(IF=1:100-500)

Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CH25H) Polyclonal Antibody, Unconjugated (bs-41322R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Lane 1: Recombinant human CH25H protein, His(bs-41322P) probed with CH25H Polyclonal Antibody, Unconjugated (bs-41322R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.