bs-4143R [Primary Antibody]
Glutamine synthetase Polyclonal Antibody
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Host: Rabbit

Target Protein: Glutamine synthetase

Immunogen Range: 285-373/373


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2752

Swiss Prot: P15104

Source: KLH conjugated synthetic peptide derived from human Glutamine syntase/Gl Syn

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). Essential for proliferation of fetal skin fibroblasts.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 42


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shikai Liu. et al. On-Demand Generation of Peroxynitrite from an Integrated Two-Dimensional System for Enhanced Tumor Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXXRead more>>
  • Zhan Xie. et al. A novel model of subretinal edema induced by DL-alpha aminoadipic acid. EXP EYE RES. 2023 Jan;:109388Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-Glutamine synthetase Polyclonal Antibody, Unconjugated (bs-4143R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


25 ug total protein per lane of various lysates (see on figure) probed with Glutamine synthetase polyclonal antibody, unconjugated (bs-25478R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.