bs-4173R

DATASHEET

Host: Rabbit

Target Protein: TWIST1 and TWIST2

Specificity: This antibody will detect TWIST1 and TWIST2

Immunogen Range: 75-160/160

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7291; 117581

Swiss Prot: Q15672; Q8WVJ9

Source: KLH conjugated synthetic peptide derived from human TWIST2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

TWIST1 and TWIST2 are basic helix-loop-helix transcriptional repressors that bind to E boxes in gene promoters, acting as master regulators in a variety of biological processes, including organogenesis, osteogenesis, cancer progression and hematopoietic cell development. Both TWIST1 and TWIST2 are found to act as Homodimers or Heterodimers with another bHLH protein. TWIST1 Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. TWIST1 also represses expression of proinflammatory cytokines such as TNFA and IL1B. TWIST2 inhibits transcriptional activation by MYOD1, MYOG, MEF2A, and MEF2C. Twist2 inhibits the premature or ectopic differentiation of preosteoblast cells during osteogenesis.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 18

Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.