bs-4182R [Primary Antibody]
RPA2 Polyclonal Antibody
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Host: Rabbit

Target Protein: RPA2

Immunogen Range: 101-200/270


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6118

Swiss Prot: P15927

Source: KLH conjugated synthetic peptide derived from human RPA32/RPA2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 29


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang HG et al. Synergistic Lethality Between PARP-trapping and Alantolactone-Induced Oxidative DNA Damage in Homologous Recombination-Proficient Cancer Cells. Oncogene . 2020 Apr;39(14):2905-2920.Read more>>
  • Wang HG et al. Synergistic Lethality Between PARP-trapping and Alantolactone-Induced Oxidative DNA Damage in Homologous Recombination-Proficient Cancer Cells. Oncogene . 2020 Apr;39(14):2905-2920.Read more>>
  • Shan Zhang. et al. Synergistic lethality between auranofin-induced oxidative DNA damage and ATR inhibition in cancer cells. LIFE SCI. 2023 Nov;332:122131Read more>>
VALIDATION IMAGES

Mouse bone/muscle lysates 30ug, probed with Anti-RPA32 Polyclonal Antibody (bs-4182R) at 1:300 overnight in 4˚C. Followed by conjugation to the secondary antibody (bs-0295G-HRP) at 1:5000 90min in RT.


Paraformaldehyde-fixed, paraffin embedded mouse small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with RPA32 Polyclonal Antibody (bs-4182R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.