bs-4187R-A350 [Conjugated Primary Antibody]
ZEB Polyclonal Antibody, ALEXA FLUOR® 350 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ZEB1/2

Specificity: Antibody will bind to Zinc finger E-box-binding homeobox 1 & 2

Immunogen Range: 211-320/1124


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6935, 9839

Swiss Prot: P37275, O60315

Source: KLH conjugated synthetic peptide derived from human ZEB1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

ZEB1 Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. ZEB2 is a Transcriptional inhibitor that binds to DNA sequence 5'-CACCT-3' in different promoters. Represses transcription of E-cadherin.

Conjugation: ALEXA FLUOR® 350

Excitation/ Emission: 346nm/442nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 124


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sasaki, Takamitsu, et al. "Significance of epithelial growth factor in the epithelial?Cmesenchymal transition of human gallbladder cancer cells."Cancer Science (2012).0Read more>>
  • Xiang, Shuai, et al. "ZEB1 Expression Is Correlated With Tumor Metastasis and Reduced Prognosis of Breast Carcinoma in Asian Patients." Cancer Investigation (2015).Read more>>
  • Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
VALIDATION IMAGES