bs-4195R [Primary Antibody]
ADAM8 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADAM8

Immunogen Range: 51-150/824


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 101

Swiss Prot: P78325

Source: KLH conjugated synthetic peptide derived from human ADAM8

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Possible involvement in extravasation of leukocytes.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)

Predicted Molecular Weight: 87


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

A431 (Positive) and HepG2 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ADAM8 Antibody (bs-4195R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).