bs-4216R [Primary Antibody]
Sphingomyelin Synthase 1 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Sphingomyelin Synthase 1

Immunogen Range: 50-100/419

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 259230

Swiss Prot: Q86VZ5

Source: KLH conjugated synthetic peptide derived from human Sphingomyelin Synthase 1

Purification: Purified by Protein A.

Storage: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.

Background: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types such as HeLa cells. Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: mouse embryo lysates Lane 2: mouse heart lysates probed with Anti Sphingomyelin Synthase 1 Polyclonal Antibody, Unconjugated (bs-4216R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 49kD. Observed band size: 49kD.


Formalin-fixed and paraffin embedded mouse brain labeled with Anti-Sphingomyelin Synthase 1 Polyclonal Antibody, Unconjugated (bs-4216R) followed by conjugation to the secondary antibody and DAB staining