bs-4236R [Primary Antibody]
ADAM17 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ADAM17

Immunogen Range: 201-300/824


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6868

Swiss Prot: P78536

Source: KLH conjugated synthetic peptide derived from human ADAM17

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 68/91


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Shalaby L et al. Role of Endothelial ADAM17 in Early Vascular Changes Associated with Diabetic Retinopathy. J Clin Med. 2020 Feb 2;9(2). pii: E400.Read more>>
  • Cyro Jos de Almeida Guardiola et al. DC-STAMP and TACE Levels are Higher in Patients with Periodontitis. Braz Dent J. Mar-Apr 2020;31(2):122-126.Read more>>
  • Ngamsri Kristian-Christos. et al. CX3CR1 Depletion Promotes the Formation of PlateletCNeutrophil Complexes and Aggravates Acute Peritonitis. Shock. 2021 Aug;56(2):287-297Read more>>
  • Haolu Sun. et al. iRhom1 rescues cognitive dysfunction in multiple sclerosis via preventing myelin injury. Genes Brain Behav. 2021 Nov;20(8):e12771Read more>>
  • Xiao, Wenyuan. et al. Robo4 is constitutively shed by ADAMs from endothelial cells and the shed Robo4 functions to inhibit Slit3-induced angiogenesis. Sci Rep-Uk. 2022 Mar;12(1):1-14Read more>>
  • Maria Consiglia Trotta. et al. Oral Administration of Vitamin D3 Prevents Corneal Damage in a Knock-Out Mouse Model of Sjögren’s Syndrome. BIOMEDICINES. 2023 Feb;11(2):616Read more>>
  • Chan Yen-Ju. et al. Blue light exposure collapses the inner blood-retinal barrier by accelerating endothelial CLDN5 degradation through the disturbance of GNAZ and the activation of ADAM17. FLUIDS BARRIERS CNS. 2023 Dec;20(1):1-16Read more>>
VALIDATION IMAGES

U937 cells probed with \tADAM17 Polyclonal Antibody, Unconjugated (bs-4236R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).