bs-42458P-Biotin

DATASHEET

Entrez Gene: 5111

Swiss Prot: P12004

Source: Recombinant human PCNA protein in E.coli with C-His. It contains the amino acid sequence of

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

Proliferating cell nuclear antigen (PCNA) is a 28kDa nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100ug,500ug,20ug

Applications: Others()

Mol. Wt.: 29.2

Species: Others

For research use only. Not intended for diagnostic or therapeutic use.