bs-4245R [Primary Antibody]
LONP1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: LONP1

Immunogen Range: 361-460/959


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 9361

Swiss Prot: P36776

Source: KLH conjugated synthetic peptide derived from human LONP1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters. Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 98


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Nie et al. Down-regulating overexpressed human Lon in cervical cancer suppresses cell proliferation and bioenergetics. (2013) PLoS.One. 8:e81084Read more>>
  • Renata R. Braga. et al. Exercise alters the mitochondrial proteostasis and induces the mitonuclear imbalance and UPR mt in the hypothalamus of mice. Sci Rep-Uk. 2021 Feb;11(1):1-13Read more>>
  • Zai, Zhuoyan. et al. Estrogen antagonizes ASIC1a-induced chondrocyte mitochondrial stress in rheumatoid arthritis. J TRANSL MED. 2022 Dec;20(1):1-14Read more>>
  • Mu?oz Vitor Rosetto. et al. Effects of short-term endurance and strength exercise in the molecular regulation of skeletal muscle in hyperinsulinemic and hyperglycemic Slc2a4+/? mice. CELL MOL LIFE SCI. 2023 May;80(5):1-16Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat lung tissue labeled with Anti-LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti- LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody


Formalin-fixed and paraffin embedded rat colon labeled with Anti- LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37°C


L1 mouse kidney lysates L2 mouse intestine lysates probed with Anti LONP1 Polyclonal Antibody, Unconjugated (bs-4245R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 98kD. Observed band size: 98kD