bs-4251R-A680 [Conjugated Primary Antibody]
PHD1 Polyclonal Antibody, ALEXA FLUOR® 680 Conjugated
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: PHD1

Immunogen Range: 321-407/407


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 112398

Source: KLH conjugated synthetic peptide derived from human PHD1/prolyl hydroxylase

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

PHD1 catalyzes the posttranslational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins and hydroxylates HIF-1 alpha at Pro-402 and Pro-564, and HIF-2 alpha. It functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitylation complex. It may play a role in cell growth regulation.

Conjugation: ALEXA FLUOR® 680

Excitation/ Emission: 679nm/702nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 44


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Loeffler, Ivonne, et al. "Heterozygosity of mitogen-activated protein kinase organizer 1 ameliorates diabetic nephropathy and suppresses epithelial-to-mesenchymal transition-like changes in db/db mice." Nephrology Dialysis Transplantation (2017).Read more>>