Host: Rabbit
Target Protein: NR0B2
Immunogen Range: 31-130/257
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8431
Swiss Prot: Q15466
Source: KLH conjugated synthetic peptide derived from human NR0B2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 28
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species:
Human
Dog
Cow
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Liu Y et al. Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD. J Nutr Biochem. 2019 Apr 4;69:53-62.Read more>>
- Shu-Yan Gao. et al. iTRAQ Quantitative Proteomic Analysis of Different Expressed Proteins and Signal Pathways in Bakuchiol-Induced Hepatotoxicity. EVID-BASED COMPL ALT. 2022 Sep 19;2022:292824Read more>>
- Jing Zhao. et al. The choleretic role of tauroursodeoxycholic?acid exacerbates alpha-naphthylisothiocyanate?induced cholestatic liver injury through the FXR/BSEP pathway. J APPL TOXICOL. 2023 FebRead more>>
- Song Guochao. et al. Potential therapeutic action of tauroursodeoxycholic acid against cholestatic liver injury via hepatic Fxr/Nrf2 and CHOP-DR5-caspase-8 pathway. CLIN SCI. 2023 Apr;137(7):561-577Read more>>
- Xiaoyue Li. et al. Dietary bile acids promote sterol metabolism, bile acids enterohepatic circulation, and apoptosis in juvenile Pacific white shrimp (Litopenaeus vannamei). ANIM FEED SCI TECH. 2023 Sep;303:11571Read more>>
Paraformaldehyde-fixed, paraffin-embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) incubation at 37°C for 20 minutes; Antibody incubation with NROB2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Small intestine tissue lysates probed with NR0B2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.