support@biossusa.com
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
Host: Rabbit
Target Protein: IL-8/CXCL8
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3576
Swiss Prot: P10145
Source: Recombinant human IL-8/CXCL8 protein
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Size: 100uL
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:100-500)
IHC-F(IHC-F=1:100-500)
Predicted Molecular Weight: 11
Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
U-87MG (H) cells were treated with or without Thapsigargin (1µM 24h), 25 μg total protein per lane of cell lysates (see on figure) probed with IL-8/CXCL8 polyclonal antibody, unconjugated (bs-44001R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-44001R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Chronic tonsillitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-44001R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-44001R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-44001R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Esophageal Cancer;Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL-8/CXCL8 Polyclonal Antibody, Unconjugated (bs-44001R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.