bs-4511R [Primary Antibody]
tubulin Beta Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: tubulin Beta

Immunogen Range: 61-160/444


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 203068

Swiss Prot: P07437

Source: KLH conjugated synthetic peptide derived from human tubulin Beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse
Rat
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013).Read more>>
  • Li, Xinxin, et al. "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signaling." Bioscience Reports (2014).Read more>>
  • Yan, Lijie, et al. "Regulator of calcineurin 1-1L protects cardiomyocytes against hypoxia-induced apoptosis via mitophagy." Journal of cardiovascular pharmacology 64.4 (2014): 310-317.Read more>>
  • 朱镕鑫, et al. "Ghrelin 信号通路在有氧运动对高胆固醇血症小鼠心肌损伤保护中的作用."Read more>>
  • Li, Xinxin, et al. "Long-term thermal manipulation in the late incubation period can inhibit breast muscle development by activating endoplasmic reticulum stress in duck (Anasplatyrhynchos domestica)." Journal of thermal biology 70.Pt B (2017): 37.Read more>>
  • Liu et al. Effects of insulin-like growth factor binding protein 3 on apoptosis of cutaneous squamous cell carcinoma cells. (2018) Onco.Targets.Ther. 11:6569-6577Read more>>
  • Sun F et al. PKA/β2-AR-Gs/Gi signaling pathway is associated with anti-inflammatory and pro-apoptotic effects of Fuzi and Banxia combination on rats subjected to pressure overload. J Ethnopharmacol. 2019 May 10;235:375-384.Read more>>
  • Fang Q et al. Evaluation of a hybrid small caliber vascular graft in rabbit model.J Thorac Cardiovasc Surg. 2019 Mar 6. pii: S0022-5223(19)30550-1.Read more>>
  • Wu MM et al. Lovastatin attenuates renal tubule‐specific ABCA1 knockout‐induced hypertension by inhibiting the epithelial sodium channel. Br J Pharmacol. 2019 Jun 21. Read more>>
  • Ma Q et al. Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Am J Transl Res. 2019 Aug 15;11(8):5008-5018. eCollection 2019.Read more>>
  • Nikonorova IA et al. Age modulates liver responses to asparaginase-induced amino acid stress in mice. J Biol Chem. 2019 Aug 14. pii: jbc.RA119.009864. Read more>>
  • Li-Hua Muet al. Antiangiogenic effects of AG36, a triterpenoid saponin from Ardisia gigantifolia stapf. J Nat Med. 2020 Sep;74(4):732-740.Read more>>
  • Haixiu Ma. et al. Photodynamic effect of chlorin e6 on cytoskeleton protein of human colon cancer SW480 cells. Photodiagn Photodyn. 2021 Mar;33:102201Read more>>
  • Mengyan Wang. et al. Analysis of the Bovine DLK1 Gene Polymorphism and Its Relation to Lipid Metabolism in Chinese Simmental. Animals-Basel. 2020 Jun;10(6):923Read more>>
  • Ge Liu. et al. Marine bacterial exopolysaccharide EPS11 inhibits migration and invasion of liver cancer cells by directly targeting collagen I. J Biol Chem. 2021 Aug;:101133Read more>>
  • Ziyu Meng. et al. Sesamin promotes apoptosis and pyroptosis via autophagy to enhance antitumour effects on murine T-cell lymphoma. J Pharmacol Sci. 2021 Nov;147:26Read more>>
  • Ping Jiang. et al. Transcriptomic Analysis of Short/Branched-Chain Acyl-Coenzyme a Dehydrogenase Knocked Out bMECs Revealed Its Regulatory Effect on Lipid Metabolism. Front Vet Sci. 2021; 8: 744287Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-BrdU(A7) Monoclonal Antibody, FITC Conjugated (bsm-0917M-FITC) at 1:200 for 40 minutes at 37°C followed by labeling Rabbit Anti-Tubulin Beta, Cy3 conjugated(bs-4511R-Cy3) 1:200, 40 minutes at 37°C


Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 50kD. Observed band size: 50kD.


Lane 1:Mouse heart lysates; Lane 2: Human MCF7 cell lysates probed with Rabbit Anti-Tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


Formalin-fixed and paraffin embedded rat brain labeled with Anti-tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Mouse ovary lysates probed with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Cerebrum lysates probed with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Uterus lysates probed with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with tubulin Beta Polyclonal Antibody, Unconjugated (bs-4511R) at 1:200 overnight at 4°C, DAB staining.


Lane 1: SiHa cell lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Mouse Cerebrum lysates; Lane 4: Rat Heart lysates ; Lane 5: Mouse Heart lysates probed with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: Mouse Cerebellum lysates; Lane 3: NIH/3T3 cell lysates; Lane 4: Mouse Cerebrum lysates ; Lane 5: HL60 cell lysates; Lane 6: Rat Cerebrum lysates; Lane 7: Rat Cerebellum lysates; Lane 8: Mouse Heart lysates probed with Beta tubulin Polyclonal Antibody, Unconjugated (bs-4511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


HL-60 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Beta tubulin Polyclonal Antibody(bs-4511R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).