bs-4538R [Primary Antibody]
TGF Beta 1+2+3 Polyclonal Antibody
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]

Host: Rabbit

Target Protein: TGF Beta 1+2+3

Specificity: This antibody will preferentially react with TGFB1, but will also detect TGFB2 (93%) and TGFB3 (86%) based upon sequence similarity.

Immunogen Range: 301-350/390

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7040

Swiss Prot: P01137

Source: KLH conjugated synthetic peptide derived from human TGF Beta 1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.


Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 12.8/44

Cross Reactive Species: Human

Predicted Cross Reactive Species: Dog
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

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  • He, Hongying, et al. "Carbamylated erythropoietin ameliorates cardiomyopathy via ERK (44/42) activation in rats with diabetic cardiomyopathy." Int J Clin Exp Med 10.10 (2017): 14213-14222.Read more>>
  • Liu Y et al. Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood.(2018)Vet Immunol Immunopathol. Nov;205:83-92. Read more>>
  • Ju B et al. miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepaticstellate cells. J Cell Mol Med. 2019 Apr 3.Read more>>
  • Li XP et al. LPS induces activation of the TLR4 pathway in fibroblasts and promotes skin scar formation through collagen I and TGF-β in skin lesions. Int J Clin Exp Pathol 2019;12(6):2121-2129.Read more>>
  • in Zeng. et al. Dietary Soy Protein Isolate Attenuates Intestinal Immunoglobulin and Mucin Expression in Young Mice Compared with Casein. Nutrients. 2020 Sep;12(9):2739Read more>>
  • Yanhong Li. et al. Therapeutic potential of human umbilical cord mesenchymal stem cells on aortic atherosclerotic plaque in a high-fat diet rabbit model. Stem Cell Res Ther. 2021 Dec;12(1):1-14Read more>>
  • Yanhong Li. et al. Allogeneic Adipose-Derived Mesenchymal Stem Cell Transplantation Alleviates Atherosclerotic Plaque by Inhibiting Ox-LDL Uptake, Inflammatory Reaction and Endothelial Damage in Rabbits. CELLS-BASEL. 2023 Jan;12(15):1936Read more>>

Rabbit spleen lysates probed with TGF beta 1+2+3 Polyclonal Antibody, Unconjugated (bs-4538R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.

Formalin-fixed and paraffin embedded rat brain labeled with Anti-TGF Beta 1+2+3 Polyclonal Antibody, Unconjugated (bs-4538) at 1:200 followed by conjugation to the secondary antibody and DAB staining

MDA-MB-231 lysates probed with TGF Beta 1+2+3 Polyclonal Antibody, unconjugated (bs-4538R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.

Mouse Stomach lysates probed with TGF Beta 1+2+3 Polyclonal Antibody, unconjugated (bs-4538R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

K562 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with TGF Beta 1+2+3 Antibody(bs-4538R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).