bs-4563R [Primary Antibody]
Bcl-2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Bcl-2

Immunogen Range: 169-211/239

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 596

Swiss Prot: P10415

Source: KLH conjugated synthetic peptide derived from human Bcl-2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Yigang, et al. "In vivo and in vitro evaluation of effects of Mg-6Zn alloy on apoptosis of common bile duct epithelial cell." Biometals: 1-14.Read more>>
  • Chen, Lina, et al. "Label-free electrochemical immunoassay of Bcl-2 protein expression on tumor cells." Talanta (2014).Read more>>
  • Huang, Guo-fu, et al. "Electroacupuncture Stimulates Remodeling of Extracellular Matrix by Inhibiting Apoptosis in a Rabbit Model of Disc Degeneration." Evidence-Based Complementary and Alternative Medicine(2015).Read more>>
  • Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016).Read more>>
  • Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.Read more>>
  • Sutrisno, Sutrisno, et al. "The effect of genistein on TGF-β signal, dysregulation of apoptosis, cyclooxygenase-2 pathway, and NF-kB pathway in mice peritoneum of endometriosis model." Middle East Fertility Society Journal (2017).Read more>>
  • Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).Read more>>
  • Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543Read more>>
  • Chen S et al. Comparative assessment of the biocompatibility and degradation behavior of Zn-3Cu and JDBM alloys used for biliary surgery. Am J Transl Res. 2020 Jan 15;12(1):19-31. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rabbit pancreas labeled with Anti-Bcl-2 Polyclonal Antibody, Unconjugated (bs-4563R) followed by conjugation to the secondary antibody and DAB staining