bs-4572R [Primary Antibody]
VEGF-A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VEGF-A

Immunogen Range: 141-180/191


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7422

Swiss Prot: P15692

Source: KLH conjugated synthetic peptide derived from human VEGF-A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 24


Cross Reactive Species: Human
Mouse
Rat
Chicken

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tao, Wang, et al. "Transplantation of bone marrow stromal cells overexpressing human vascular endothelial growth factor 165 enhances tissue repair in a rat model of radiation-induced injury." Chinese Medical Journal 127.6 (2014): 1093-1099.Read more>>
  • Fetoni, Anna Rita, et al. "Grafting and early expression of growth factors from adipose-derived stem cells transplanted into the cochlea, in a guinea pig model of acoustic trauma."Frontiers in Cellular Neuroscience 8 (2014): 334.Read more>>
  • Fetoni, A. R., et al. "The redox protein p66shc mediates cochlear vascular dysfunction and transient noise-induced hearing loss." Scientific Reports 6 (2016): 25450.Read more>>
  • Hedeen, Heather A. "A Kinetic Study of anti-VEGF-A Polyclonal Antibodies and anti-VEGF-A ssDNA Aptamers." (2012).Read more>>
  • Tian et al. Mechanisms of neuroprotection from hypoxia-ischemia (HI) brain injury by up-regulation of cytoglobin (CYGB) in a neonatal rat model. (2013) J.Biol.Che. 288:15988-6003Read more>>
  • Shi et al. Peptide Lv augments L-type voltage-gated calcium channels through vascular endothelial growth factor receptor 2 (VEGFR2) signaling. (2015) Biochim.Biophys.Acta. 1853:1154-64Read more>>
  • Tian Jing. et al. linc00958/miR-185-5p/RSF-1 modulates cisplatin resistance and angiogenesis through AKT1/GSK3/VEGFA pathway in cervical cancer. REPROD BIOL ENDOCRIN. 2022 Dec;20(1):1-12Read more>>
  • Yuksel Olgun. et al. The Effect of Bevacizumab and Propranolol on Nasal Polyposis. INT J CLIN PRACT. 2022 Oct 12;2022:6174664Read more>>
  • Lin Li. et al. Overexpression and potential roles of midkine via regulation of vascular endothelial growth factor A in psoriasis. EXP DERMATOL. 2023 MayRead more>>
  • Wenjun Chen. et al. Salvianolate Ameliorates Renal Damage Induced by C-BSA in Membranous Nephropathy Rats Through Inhibiting Hypercoagulable State and Alleviating Podocyte Injury. PHARMACOGN MAG. ;()Read more>>
VALIDATION IMAGES

Antigen: bs-4572P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti VEGF-A Polyclonal Antibody, Unconjugated (bs-4572R) at 1:200 followed by conjugation to the secondary antibody and DAB staining