bs-4590R [Primary Antibody]
PPAR Gamma Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PPAR Gamma

Immunogen Range: 315-420/475


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5468

Swiss Prot: P37231

Source: KLH conjugated synthetic peptide derived from human PPAR Gamma

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Nuclear receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the nuclear receptor binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis. ARF6 acts as a key regulator of the tissue-specific adipocyte P2 (aP2) enhancer. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated proinflammatory responses. Plays a role in the regulation of cardiovascular circadian rhythms by regulating the transcription of ARNTL/BMAL1 in the blood vessels (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 57


Cross Reactive Species: Human
Mouse
Rat
Goat

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sun, Chao, et al. "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." BioMed Research International 2015 (2015).Read more>>
  • Li et al. Valproate Attenuates Nitroglycerin-Induced Trigeminovascular Activation by Preserving Mitochondrial Function in a Rat Model of Migraine. (2016) Med.Sci.Monit. 22:3229-37Read more>>
  • Dervishi I et al. Protein-protein interactions reveal key canonical pathways, upstream regulators, interactome domains, and novel targets in ALS.(2018) Sci Rep 8(1):14732. Read more>>
  • Dervishi et al. Protein-protein interactions reveal key canonical pathways, upstream regulators, interactome domains, and novel targets in ALS. (2018) Sci.Rep. 8:14732Read more>>
  • Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193.Read more>>
  • Chen HJ et al. Nicotine induces a dual effect on the beige-like phenotype in adipocytes. Arch Biol Sci. 2019;71(3):533-540Read more>>
  • Qi H et al. MSTN attenuates cardiac hypertrophy through inhibition of excessive cardiac autophagy by blocking AMPK/mTOR and miR-128/PPARγ/NF-κB signaling pathways. Mol Ther Nucleic Acids. 2019 Dec 14;19:507-522. Read more>>
  • Tan W et al. Pyrazinamide alleviates rifampin-induced steatohepatitis in mice by regulating the activities of cholesterol-activated 7-hydroxylase and lipoprotein lipase. Eur J Pharm Sci. 2020 Aug 1;151:105402.Read more>>
  • Shogo Kumagaiet al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells. Immunity. 2020 Jul 14;53(1):187-203.e8.Read more>>
  • Qianqian Dong. et al. Tetrahydroxystilbene glycoside improves endothelial dysfunction and hypertension in obese rats: the role of omentin-1. Biochem Pharmacol. 2021 Feb;:114489Read more>>
  • Ting Zhang. et al. Sea buckthorn ( Hippophae rhamnoides L.) oil enhances proliferation, adipocytes differentiation and insulin sensitivity in 3T3-L1 cells. Food Sci Biotechnol. 2020 Nov;29(11):1511-1518Read more>>
  • Xiang Yu. et al. Isolation and Identification of Bovine Preadipocytes and Screening of MicroRNAs Associated with Adipogenesis. Animals-Basel. 2020 May;10(5):818Read more>>
  • Shuo Geng. et al. Resolving monocytes generated through TRAM deletion attenuate atherosclerosis. Jci Insight. 2021 Sep;():149651Read more>>
  • Ningfei Ji. et al. SARS-CoV-2 in the pancreas and the impaired islet function in COVID-19 patients. 2022 Mar 28Read more>>
  • Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022Read more>>
  • Li Xu. et al. Fenpropathrin increases gliquidone absorption via causing damage to the integrity of intestinal barrier. ECOTOX ENVIRON SAFE. 2022 Sep;242:113882Read more>>
  • Yang Huan. et al. Effects of maternal undernutrition during late pregnancy on the regulatory factors involved in growth and development in ovine fetal perirenal brown adipose tissue. Animal Bioscience. 2022 Jul;35(7):1010-102Read more>>
  • Hongya Wu. et al. The Protective Effects of Iron Free Lactoferrin on Lipopolysaccharide-Induced Intestinal Inflammatory Injury via Modulating the NF-κB/PPAR Signaling Pathway. FOODS. 2022 Jan;11(21):3378Read more>>
  • Zhe Sui. et al. Ginsenoside Rg3 has effects comparable to those of ginsenoside re on diabetic kidney disease prevention in db/db mice by regulating inflammation, fibrosis and PPAR. MOL MED REP. 2023 Apr;27(4):1-1Read more>>
  • Pan-Pan Guo. et al. Overexpression of DGAT2 Regulates the Differentiation of Bovine Preadipocytes. ANIMALS. 2023 Jan;13(7):1195Read more>>
  • Hao Yu. et al. Tumor necrosis factor- reduces adiponectin production by decreasing transcriptional activity of peroxisome proliferator-activated receptor- in calf adipocytes. J DAIRY SCI. 2023 JuRead more>>
  • Jing Zhang. et al. Dietary supplementation of sodium butyrate enhances lactation performance by promoting nutrient digestion and mammary gland development in dairy cows. ANIM NUTR. 2023 SepRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat aorta tissue labeled with Anti-PPAR Gamma Polyclonal Antibody, Unconjugated (bs-4590R) at 1:300 followed by conjugation to the secondary antibody


U251 cells probed with PPAR Gamma Polyclonal Antibody, Unconjugated (bs-4590R) for 30 minutes followed by incubation with a conjugated secondary (FITC Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Mouse Placenta lysates probed with PPAR Gamma Polyclonal Antibody, Unconjugated (bs-4590R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Lane 1: Human A549 cell lysates; Lane 2: Rat Adipocyte tissue lysates; Lane 3: Mouse Large intestine tissue lysates; Lane 4: Mouse Lung tissue lysates probed with PPAR gamma Polyclonal Antibody, Unconjugated (bs-4590R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.