bs-4595R [Primary Antibody]
Collagen IV Polyclonal Antibody
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Host: Rabbit

Target Protein: Collagen IV

Immunogen Range: 1571-1669/1669


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1282

Swiss Prot: P02462

Source: KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 165


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Belair, David G., et al. "Engineering human cell spheroids to model embryonic tissue fusion in vitro." PloS one 12.9 (2017): e0184155.Read more>>
  • Bao et al. Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold. (2016) Int.J.Mol.Me. 38:457-65Read more>>
  • Wu et al. MicroRNA-27a Induces Mesangial Cell Injury by Targeting of PPARγ, and its In Vivo Knockdown Prevents Progression of Diabetic Nephropathy. (2016) Sci.Rep. 6:26072Read more>>
  • Song et al. Extracellular diffusion quantified by magnetic resonance imaging during rat C6 glioma cell progression. (2017) Braz.J.Med.Biol.Res. 50:e5404Read more>>
  • Gao X et al. Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Pathol Res Pract. 2020 Mar;216(3):152831. Read more>>
  • H-J Wang. et al. MiR-32-5p knockdown inhibits epithelial to mesenchymal transition and renal fibrosis by targeting SMAD7 in diabetic nephropathy:. Hum Exp Toxicol. 0;()Read more>>
  • Siwen Zhang. et al. Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency. Stem Cell Res Ther. 2021 Dec;12(1):1-16Read more>>
  • Yan Wang. et al. Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation. Phytomedicine. 2022 Jun;100:154068Read more>>
  • Xin Liu. et al. Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury. BIOMATERIALS. 2022 Oct;:121862Read more>>
  • Ali Simsek. et al. Histopathological and Immunohistochemical Effects of Tarantula cubensis Extract on Mucosal Healing in Rats. J VET DENT. ;()Read more>>
  • Asci Halil. et al. Combined Pulsed Magnetic Field and Radiofrequency Electromagnetic Field Enhances MMP-9, Collagen-4, VEGF Synthesis to Improve Wound Healing Via Hif-1/eNOS Pathway. AESTHET PLAST SURG. 2023 Jun;:1-12Read more>>
  • Cynthia J. Wolf. et al. An improved multicellular human organoid model for the study of chemical effects on palatal fusion. BIRTH DEFECTS RES. 2023 AugRead more>>
  • Libo Liu. et al. Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Alleviate Diabetic Kidney Disease in Rats by Inhibiting Apoptosis and Inflammation. FRONT BIOSCI-LANDMRK. 2023 Sep;28(9):203Read more>>
  • Zhu Yuan-Zheng. et al. Genome-wide search links senescence-associated secretory proteins with susceptibility for coronary artery disease in mouse and human. J GERONTOL A-BIOL. 2024 FebRead more>>
  • Demircan Volkan. et al. Evaluation of therapeutic use of a combination of pentoxifylline and vitamin E in radiation-induced renal fibrosis. SCI REP-UK. 2024 Mar;14(1):1-14Read more>>
  • Sun Qianqian. et al. Single-cell transcriptome analysis reveals the regulatory functions of islet exocrine cells after short-time obesogenic diet. ENDOCRINE. 2024 May;:1-11Read more>>
  • Liu Zao-Ling. et al. WGCNA reveals a biomarker for cancer-associated fibroblasts to predict prognosis in cervical cancer. J CHIN MED ASSOC. 2024 Jul;:10.1097/JCMA.0000000000001129Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei


Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (bs-4595R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in EDTA buffer (pH9.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (bs-4595R) at 1:600 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Collagen IV) Polyclonal Antibody, Unconjugated (bs-4595R) at 1:300 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.