Host: Rabbit
Target Protein: Collagen 4
Immunogen Range: 1571-1669/1669
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1282
Swiss Prot: P02462
Source: KLH conjugated synthetic peptide derived from human Collagen alpha-1(IV) chain
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 165
Cross Reactive Species:
Human
Mouse
Rat
Pig
Predicted Cross Reactive Species:
Dog
Cow
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Belair, David G., et al. "Engineering human cell spheroids to model embryonic tissue fusion in vitro." PloS one 12.9 (2017): e0184155.Read more>>
- Bao et al. Enhanced hepatic differentiation of rat bone marrow-derived mesenchymal stem cells in spheroidal aggregate culture on a decellularized liver scaffold. (2016) Int.J.Mol.Me. 38:457-65Read more>>
- Wu et al. MicroRNA-27a Induces Mesangial Cell Injury by Targeting of PPARγ, and its In Vivo Knockdown Prevents Progression of Diabetic Nephropathy. (2016) Sci.Rep. 6:26072Read more>>
- Song et al. Extracellular diffusion quantified by magnetic resonance imaging during rat C6 glioma cell progression. (2017) Braz.J.Med.Biol.Res. 50:e5404Read more>>
- Gao X et al. Alteration and prognostic values of collagen gene expression in patients with gastric cancer under different treatments. Pathol Res Pract. 2020 Mar;216(3):152831. Read more>>
- H-J Wang. et al. MiR-32-5p knockdown inhibits epithelial to mesenchymal transition and renal fibrosis by targeting SMAD7 in diabetic nephropathy:. Hum Exp Toxicol. 0;()Read more>>
- Siwen Zhang. et al. Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency. Stem Cell Res Ther. 2021 Dec;12(1):1-16Read more>>
- Yan Wang. et al. Geniposide alleviates VEGF-induced angiogenesis by inhibiting VEGFR2/PKC/ERK1/2-mediated SphK1 translocation. Phytomedicine. 2022 Jun;100:154068Read more>>
- Xin Liu. et al. Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury. BIOMATERIALS. 2022 Oct;:121862Read more>>
- Ali Simsek. et al. Histopathological and Immunohistochemical Effects of Tarantula cubensis Extract on Mucosal Healing in Rats. J VET DENT. ;()Read more>>
- Asci Halil. et al. Combined Pulsed Magnetic Field and Radiofrequency Electromagnetic Field Enhances MMP-9, Collagen-4, VEGF Synthesis to Improve Wound Healing Via Hif-1/eNOS Pathway. AESTHET PLAST SURG. 2023 Jun;:1-12Read more>>
- Cynthia J. Wolf. et al. An improved multicellular human organoid model for the study of chemical effects on palatal fusion. BIRTH DEFECTS RES. 2023 AugRead more>>
- Libo Liu. et al. Bone Marrow Mesenchymal Stem Cell-Derived Exosomes Alleviate Diabetic Kidney Disease in Rats by Inhibiting Apoptosis and Inflammation. FRONT BIOSCI-LANDMRK. 2023 Sep;28(9):203Read more>>
- Zhu Yuan-Zheng. et al. Genome-wide search links senescence-associated secretory proteins with susceptibility for coronary artery disease in mouse and human. J GERONTOL A-BIOL. 2024 FebRead more>>
Formalin-fixed and paraffin embedded rat kidney tissue labeled with Anti-Collagen IV Polyclonal Antibody, Unconjugated (bs-4595R) at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, PE conjugated (bs-0295G-PE) used at 1:200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei
Lane 1: Recombinant human Collagen IV protein, N-His(bs-42306P) probed with Collagen IV Polyclonal Antibody, Unconjugated (bs-4595R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.