bs-46003P-Biotin [Conjugated Protein]
Recombinant SARS-CoV-2 Spike RBD, Biotin Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Swiss Prot: QHD43416.1

Source: Recombinant SARS-CoV-2 Spike RBD protein is expressed in HEK293 with His-Avi. It contains the amino acid sequence of Arg319-Asn532.

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensinconverting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Endotoxin: Less than 1EU per ug by the LAL method.

Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Sequence: Arg319-Asn532

Expression: HEK293

Tag: His-Avi

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 1mg,100ug

Applications: WB()
ELISA()
HPLC()
SDS-PAGE()

Mol. Wt.: 27


Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Biotinylated Recombinant SARS-CoV-2 S protein RBD on Tris-Bis PAGE under reduced condition. The purity is great than 95%.




The purity of Biotinylated SARS-CoV-2 S protein RBD is greater than 95% as determined by SEC-HPLC.




Immobilized n-COV RBD at 1ug/ml (100ul/Well). Dose-response curve for Biotinylated ACE2 with the EC50 of 0.1ug/ml determined by ELISA.