bs-46004P-Biotin [Conjugated Protein]
Recombinant SARS-CoV-2 Spike S1, Biotin Conjugated
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DATASHEET

Swiss Prot: QHD43416.1

Source: Recombinant SARS-CoV-2 Spike S1 protein is expressed in HEK293 with His-Avi. It contains the amino acid sequence of Gln 14-Arg 683.

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensinconverting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Sequence: Gln 14-Arg 683

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 1mg, 100ug

Applications: WB(1:300-5000)
ELISA(1:500-1000)
HPLC(N/A)

Predicted Molecular Weight: 73


Cross Reactive Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Biotinylated Recombinant SARS-CoV-2 Spike S1 protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.


The purity of biotinylated SARS-CoV-2 Spikee S1 is greater than 95% as determined by SEC-HPLC.


Immobilized biotinylated n-COV S1, His Tag at 1ug/ml (100ul/Well). Dose-response curve for ACE2, Fc tag with the EC50 of 81ng/ml determined by ELISA.