bs-46006P [Protein]
Recombinant SARS-CoV-2 Spike S1
www.biossusa.com
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DATASHEET

Swiss Prot: QHD43416.1

Source: SARS-CoV-2 Spike S1 is expressed from mammalian with a Fc tag and Avi at the C-terminal. It contains Gln 14-Arg 683.

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensinconverting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Endotoxin: Less than 1EU per ug by the LAL method.

Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Sequence: Gln 14-Arg 683

Expression: HEK293

Tag: Fc-Avi

Size: 1mg,100ug

Mol. Wt.: The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140KDa based on Bis-Tris PAGE result.

Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Recombinant SARS-CoV-2 Spike S1 protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.




The purity of SARS-CoV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.




Immobilized biotinylated ACE2, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Spike S1, Fc tag with the EC50 of 0.1ug/ml determined by ELISA.