bs-46012P [Protein]
Recombinant SARS-COV-2 Spike S1 (N501Y) Protein
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Entrez Gene: YP_009724390.1

Source: Recombinant SARS-COV-2 Spike S1 (N501Y) Protein protein is expressed in HEK293 with His tag. It contains the amino acid sequence of Val16-Arg685 (N501Y).

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

A new variant of SARS-CoV-2 is spreading in the UK and is rapidly becoming a global threat. It is characterized by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2).

Endotoxin: Less than 1EU per ug by the LAL method.

Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Sequence: Val16-Arg685 (N501Y)

Expression: HEK293

Tag: His tag

Size: 1mg,100ug

Applications: WB()
ELISA()
HPLC()

Mol. Wt.: 129


Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

SARS-CoV-2 Spike S1 (N501Y) Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.




The purity of SARS-COV-2 Spike S1 (N501Y) Protein is greater than 95% as determined by SEC-HPLC.




Immobilized SARS-CoV-2 Spike S1 (N501Y) Protein, His Tag at 0.5ug/ml (100ul/Well). Dose-response curve for ACE2, Fc tag with the EC50 of 80.3ng/ml determined by ELISA.