bs-46014P [Protein]
Recombinant SARS-COV-2 Spike S Trimer (D614G) Protein
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DATASHEET

Entrez Gene: QHD43416.1

Source: Recombinant SARS-COV-2 Spike S Trimer (D614G) Protein protein is expressed in HEK293 with His-Avi. It contains the amino acid sequence of Val16-Glu1188 (D614G).

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

The SARS-CoV-2 spike (S) protein variant D614G supplanted the ancestral virus worldwide, reaching near fixation in a matter of months. Recently, that D614G was been found more infectious than the ancestral form on human lung cells, colon cells, and on cells rendered permissive by ectopic expression of human ACE2 or of ACE2 orthologs from various mammals, including Chinese rufous horseshoe bat and Malayan pangolin.

Endotoxin: Less than 1EU per ug by the LAL method.

Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Sequence: Val16-Glu1188 (D614G)

Expression: HEK293

Tag: His-Avi

Size: 1mg,100ug

Applications: WB()
ELISA()
HPLC()

Mol. Wt.: 136


Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Recombinant SARS-COV-2 Spike S Trimer (D614G) Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.




The purity of SARS-COV-2 Spike S Trimer (D614G) Protein is greater than 95% as determined by SEC-HPLC.




Immobilized SARS-COV-2 Spike S (D614G) Trimer, His Tag at 1ug/ml (100ul/Well). Dose-response curve for ACE2, Fc tag with the EC50 of 0.1ug/ml determined by ELISA.