bs-46018P [Protein]
Recombinant SARS-COV-2 Spike RBD (N501Y, K417N, E484K) Protein
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Entrez Gene: QHD43416.1

Source: SARS-COV-2 Spike RBD (N501Y, K417N, E484K) protein is expressed from mammalian with a His-tag at the C-terminal. 

Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.

Storage: The product should be stored at -70°C or -20°C.

Background:

SARS-CoV-2 exploits angiotensin-converting enzyme 2 (ACE2) as a receptor to invade cells. It has been reported that the UK and South African strains may have higher transmission capabilities, eventually in part due to amino acid substitutions on the SARS-CoV-2 Spike protein. The results of a study show the N501Y replacement in this region of the interface (present in both the UK and South African strains) should be favorable for the interaction with ACE2, while the K417N and E484K substitutions (South African strain) would seem neutral or even unfavorable.

Endotoxin: Less than 1EU per ug by the LAL method.

Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC

Sequence: Arg319-Phe541 (N501Y, K417N, E484K)

Expression: HEK293

Tag: His tag

Size: 1mg,100ug

Mol. Wt.: The protein has a predicted MW of 26.2kDa. Due to glycosylation, the protein migrates to 36-10kDa based on Bis-Tris PAGE result.


Species: SARS-CoV-2

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Recombinant SARS-CoV-2 Spike RBD (N501Y, K417N, E484K) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.




The purity of SARS-CoV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.




Immobilized SARS-CoV-2 Spike RBD (N501Y, K417N, E484K), at 0.5ug/ml (100ul/Well). Dose-response curve for ACE2, hFc tag with the EC50 of 0.48ug/ml determined by ELISA.