Entrez Gene: QHD43416.1
Source: SARS-COV-2 Spike RBD (N501Y, K417N, E484K) protein is expressed from mammalian with a His-tag at the C-terminal.
Storage Buffer: Lyophilized from 0.22um filtered solution in 20mM PB (pH 7.4). 5% trehalose is added as a protectant before lyophilization.
Storage: The product should be stored at -70°C or -20°C.
Endotoxin: Less than 1EU per ug by the LAL method.
Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Sequence: Arg319-Phe541 (N501Y, K417N, E484K)
Tag: His tag
Mol. Wt.: The protein has a predicted MW of 26.2kDa. Due to glycosylation, the protein migrates to 36-10kDa based on Bis-Tris PAGE result.
For research use only. Not intended for diagnostic or therapeutic use.
Recombinant SARS-CoV-2 Spike RBD (N501Y, K417N, E484K) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
The purity of SARS-CoV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.
Immobilized SARS-CoV-2 Spike RBD (N501Y, K417N, E484K), at 0.5ug/ml (100ul/Well). Dose-response curve for ACE2, hFc tag with the EC50 of 0.48ug/ml determined by ELISA.