bs-4609R
[Primary Antibody]
CD105 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: CD105
Immunogen Range: 58-110/625
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2022
Swiss Prot: P17813
Source: KLH conjugated synthetic peptide derived from human CD105
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Major glycoprotein of vascular endothelium. Involved in the regulation of angiogenesis. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. Acts as TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Required for GDF2/BMP9 signaling through SMAD1 in endothelial cells and modulates TGF-beta1 signaling through SMAD3.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 70
Cross Reactive Species:
Human
Mouse
Rat
Dog
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Gao, Xin, et al. "Identification of Rat Respiratory Mucosa Stem Cells and Comparison of the Early Neural Differentiation Potential with the Bone Marrow Mesenchymal Stem Cells In Vitro." Cellular and Molecular Neurobiology: 1-12.Read more>>
- Wang, Kai, et al. "Over-expression of Mash1 improves the GABAergic differentiation of bone marrow mesenchymal stem cells< i> in vitro</i>." Brain Research Bulletin (2013).Read more>>
- Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).Read more>>
- Gao, Qian, et al. "Expression pattern of embryonic stem cell markers in DFAT cells and ADSCs." Molecular biology reports 39.5 (2012): 5791-5804.Read more>>
- Gierloff, M., et al. "In vitro comparison of different carrier materials with rat bone marrow MSCs." Clinical Oral Investigations (2013): 1-13.Read more>>
- Zhao, Min, et al. "Placental expression of VEGF is increased in pregnancies with hydatidiform mole: Possible association with developing very early onset preeclampsia." Early Human Development (2013).Read more>>
- Bidkhori, Hamid Reza, et al. "Chemically primed bone-marrow derived mesenchymal stem cells show enhanced expression of chemokine receptors contributed to their migration capability." Iranian Journal of Basic Medical Sciences 19.1 (2016): 14-19.Read more>>
- Gao, Xin, et al. "Identification of rat respiratory Mucosa stem cells and comparison of the early neural differentiation potential with the bone marrow mesenchymal stem cells in vitro." Cellular and molecular neurobiology 34.2 (2014): 257-268Read more>>
- Wang, Zheng, et al. "Microparticle levels after arterial injury and NO therapy in diabetes." Journal of Surgical Research 200.2 (2016): 722-731.Read more>>
- Heirani-Tabasi, Asieh, et al. "Chemokine Receptors Expression in MSCs: Comparative Analysis in Different Sources and Passages." c14.5 (2017): 605-615.Read more>>
- Bach et al. Biologic canine and human intervertebral disc repair by notochordal cell-derived matrix: from bench towards bedside. (2018) Oncotarget. 9:26507-26526Read more>>
- Shao F et al. Multiboosting of cancer immunotherapy by a core-shell delivery system. Mol Pharm. 2020 Jan 6;17(1):338-348.Read more>>
- Ise H et al. Improved Isolation of Mesenchymal Stem Cells Based on Interactions between N-Acetylglucosamine-Bearing Polymers and Cell-Surface Vimentin. Stem Cells International, 2019, 1–13.Read more>>
- Gae Won Parket al. Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns. Sci Rep. 2020 Jul 24;10(1):12448.Read more>>
- Keiko M et al. Cultured Neonatal Rat Cardiomyocytes Continue Beating Through Upregulation of CTGF Gene ExpressionJ Nippon Med Sch.2020;87(5):268-276.Read more>>
- Ruijing Zhao. et al. Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. J Control Release. 2022 May;345:214Read more>>
- Park, Hyeri. et al. Increased phosphatase regenerating liver-1 trigger vascular remodeling in injured ovary via platelet-derived growth factor signaling pathway. Stem Cell Res Ther. 2022 Dec;13(1):1-16Read more>>
- Enoch Odame Anto. et al. Placental lesions and differential expression of pro-and anti-angiogenic growth mediators and oxidative DNA damage marker in placentae of Ghanaian suboptimal and optimal health status pregnant women who later developed preeclampsia. Plos One. 2022 Mar;17(3):e0265717Read more>>
- Jinpu Wei. et al. Biological characteristics of two mesenchymal stem cell cultures isolated from the umbilical cord and adipose tissue of a neonatal common hippo (Hippopotamus amphibius). TISSUE CELL. 2022 Aug;77:101862Read more>>
- Ziya Yurtal. et al. Protective and Therapeutic Effects of Bovine Amniotic Fluids Collected in Different Trimesters on The Epidural Fibrosis After Experimental Laminectomy in Rats. WORLD NEUROSURG. 2023 JaRead more>>
VALIDATION IMAGES
L1 rat brain, L2 rat heart lysates probed (bs-4609R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 70kDa.
Formalin-fixed and paraffin embedded rat pancreas tissue labeled with Anti-CD105 Polyclonal Antibody (bs-4609R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Image provided by One World Lab validation program. U138 cells probed with Rabbit Rabbit Anti-CD105 Polyclonal Antibody (bs-4609R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\n
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD105) Polyclonal Antibody, Unconjugated (bs-4609R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.