bs-4615R [Primary Antibody]
ICAM1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ICAM1

Immunogen Range: 451-505/532


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3383

Source: KLH conjugated synthetic peptide derived from human CD54/ICAM-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 56


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xie et al. Evaluation of Liver Ischemia-Reperfusion Injury in Rabbits Using a Nanoscale Ultrasound Contrast Agent Targeting ICAM-1. (2016) PLoS.One. 11:e0153805Read more>>
  • Hou J et al. The expression of macrophage migration inhibitory factor and intercellular adhesion molecule-1 in rats with periodontitis and atherosclerosis. Arch Oral Biol. 2019 Aug 10;107:104513. Read more>>
  • Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096Read more>>
  • Leiming Sun. et al. Isoliquiritigenin attenuates acute renal injury through suppressing oxidative stress, fibrosis and JAK2/STAT3 pathway in streptozotocin-induced diabetic rats. Bioengineered. 2021;12(2):11188-112Read more>>
  • Xin-Sen Chen. et al. Losartan attenuates sepsis-induced cardiomyopathy by regulating macrophage polarization via TLR4-mediated NF-B and MAPK signaling. PHARMACOL RES. 2022 Nov;185:106473Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rabbit carotid artery tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody, Unconjugated(bs-4615R) at 1:400 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Human A431 cell lysates; Lane 2: Human HUVEC cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human Siha cell lysates; Lane 5: Human U937 cell lysates probed with ICAM1 Polyclonal Antibody, Unconjugated (bs-4615R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C