bs-4616R [Primary Antibody]
ICAM1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ICAM1

Immunogen Range: 251-300/532


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3383

Swiss Prot: P05362

Source: KLH conjugated synthetic peptide derived from human CD54/ICAM-1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 56


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-ICAM1 Polyclonal Antibody (bs-4616R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


MCF-7 cell lysates probed with Anti-ICAM1 Polyclonal Antibody, Unconjugated (bs-4616R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD54/ICAM-1 Polyclonal Antibody, Unconjugated (bs-4616R) at 1:200 followed by conjugation to the secondary antibody and DAB staining