DATASHEET
Host:
Rabbit
Target Protein:
ICAM1
Immunogen Range:
251-300/532
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
3383
Swiss Prot:
P05362
Source:
KLH conjugated synthetic peptide derived from human CD54/ICAM-1
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. In case of rhinovirus infection acts as a cellular receptor for the virus.
VALIDATION IMAGES
Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-ICAM1 Polyclonal Antibody (bs-4616R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
MCF-7 cell lysates probed with Anti-ICAM1 Polyclonal Antibody, Unconjugated (bs-4616R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 90min in 37˚C.
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CD54/ICAM-1 Polyclonal Antibody, Unconjugated (bs-4616R) at 1:200 followed by conjugation to the secondary antibody and DAB staining