bs-4630R [Primary Antibody]
ROCK1(Thr455/Ser456) Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ROCK1 Thr455 + Ser456

Immunogen Range: 440-465/1354

Modification Site: Thr455/Ser456

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6093

Swiss Prot: Q13464

Source: KLH conjugated synthetic phosphopeptide derived from human ROCK1 around the phosphorylation site of Thr455/Ser456

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).Read more>>
  • Lin, Ping-Yuan, et al. "RhoA/ROCK1 regulates Avian Reovirus S1133-induced switch from autophagy to apoptosis." BMC veterinary research 11.1 (2015): 103.Read more>>
  • Choi et al. GM-CSF reduces expression of chondroitin sulfate proteoglycan (CSPG) core proteins in TGF-β-treated primary astrocytes. (2014) BMB.Re. 47:679-84Read more>>
  • Kim et al. Up-regulation of neogenin-1 increases cell proliferation and motility in gastric cancer. (2014) Oncotarget. 5:3386-98Read more>>
  • Kusuyama J et al. Low intensity pulsed ultrasound (LIPUS) maintains osteogenic potency by the increasedexpression and stability of Nanog through spleen tyrosine kinase (Syk) activation. Cell Signal. 2019 Jun 19;62:109345. Read more>>
  • Zhou Y et al. ROCK2 Confers Acquired Gemcitabine Resistance in Pancreatic Cancer Cells by Upregulating Transcription Factor ZEB1. Cancers (Basel). 2019 Nov 27;11(12). pii: E1881. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-phospho-ROCK1(Thr455/Ser456) Polyclonal Antibody, Unconjugated (bs-4630R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rat heart labeled with Anti-phospho-ROCK1(Thr455/Ser456) Polyclonal Antibody, Unconjugated (bs-4630R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Mouse Cerebrum lysates probed with ROCK1(Thr455+Ser456) Polyclonal Antibody, Unconjugated (bs-4630R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.