bs-4690R [Primary Antibody]
CD142 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD142

Immunogen Range: 32-100/295


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2152

Swiss Prot: P13726

Source: KLH conjugated synthetic peptide derived from human CD142

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Cow
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Liu, Shuchuan, et al. "Procoagulant activity and cellular origin of microparticles in human amniotic fluid." Thrombosis Research (2014).Read more>>
  • Tian, Ye, et al. "Brain-derived microparticles induce systemic coagulation in a murine model of traumatic brain injury." Blood (2015): blood-2014.Read more>>
  • Gleeson, Birgitta M., et al. "Bone marrow‐derived mesenchymal stem cells have innate procoagulant activity and cause microvascular obstruction following intracoronary delivery: Amelioration by anti‐thrombin therapy." STEM CELLS (2015).Read more>>
  • Milano, M., et al. "Particulate matter phagocytosis induces tissue factor in differentiating macrophages." Journal of Applied Toxicology 36.1 (2016): 151-160Read more>>
  • Kim JE, Yoo HJ, Gu JY, Kim HK (2016) Histones Induce the Procoagulant Phenotype of Endothelial Cells through Tissue Factor Up-Regulation and Thrombomodulin Down-Regulation. PLoS ONE 11(6): e0156763.Read more>>
  • Yoo, Hyun Ju, et al. "Porcine endothelium induces DNA–histone complex formation in human whole blood: a harmful effect of histone on coagulation and endothelial activation." Xenotransplantation (2016).Read more>>
  • Fletcher, Russell B., et al. "Deconstructing Olfactory Stem Cell Trajectories at Single-Cell Resolution." Cell Stem Cell (2017).Read more>>
  • Natalia Marcinczyk. et al. Multidirectional Effects of Tormentil Extract on Hemostasis in Experimental Diabetes. Front Pharmacol. 2021; 12: 682987Read more>>
  • Wang Shijun. et al. Investigations on the Changes of Serum Proteins in Rabbits after Trimeresurus stejnegeri Venom Injection via Mass Spectrometry-Based Proteomics. EVID-BASED COMPL ALT. 2022;2022:9239662Read more>>
VALIDATION IMAGES

Image provided by the Independent Validation Program,badge number 029589:Formalin-fixed and paraffin embedded mouse lung labeled with Anti-CD142 Polyclonal Antibody, Unconjugated (bs-4690R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat pancreas labeled with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (bs-4690RR) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: rat brain lysates Lane 2: rat liver lysates probed with Anti Tissue factor/CD142/F3 Polyclonal Antibody, Unconjugated (bs-4690R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band


Lane 1: Mouse Lung tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Human A431 cell lysates; Lane 4: Human MDA-MB-231 cell lysates; Lane 5: Human SiHa cell lysates probed with Tissue factor Polyclonal Antibody, Unconjugated (bs-4690R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.