bs-4707R [Primary Antibody]
Acetyl Histone H4(K17) Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Acetyl Histone H4K17

Modification Site: K17

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 121504

Swiss Prot: P62805

Source: KLH conjugated synthetic peptide derived from human Acetyl-Histone H4(K17)

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 11


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Dog
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Acetyl-Histone H4(K16) Polyclonal Antibody, Unconjugated (bs-4707R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Image kindly submitted by Piotr Mamczur as part of the free sample program. Immunofluorescent localization of acetylated histone H4 in mouse squamous cell cancer (KLN-205 cell line) with BS-4707R (1:20) antibody and FITC-labeled secondary antibodies (1:2000). The nuclei were stained with DAPI.