Host: Rabbit
Target Protein: GDF9
Immunogen Range: 301-400/454
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2661
Swiss Prot: O60383
Source: KLH conjugated synthetic peptide derived from human GDF9
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 15
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Sheep
Pig
Horse
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Pinar Kacamak. et al. The Effect of Isotretinoin on Oocyte Maturation in Adolescent Female Rats. Gynecol Obstet Inves. 2020;85(4):327-335Read more>>
- Huihui Wang. et al. Regulation of GDF9 and CDKN1B expression in Tibetan sheep testes during different stages of maturity. Gene Expr Patterns. 2021 Nov;:119218Read more>>
- F?nd?k Damla Gl. et al. Decreased growth differentiation factor 9, bone morphogenetic protein 15, and forkhead box O3a expressions in the ovary via ulipristal acetate. Revista da Associacao Medica Brasileira. 2023 Aug;69:e20230381Read more>>
Paraformaldehyde-fixed, paraffin embedded rat ovary; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with GDF9 Polyclonal Antibody, Unconjugated (bs-4720R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Lane 1: Mouse Ovary cell lysates probed with GDF9 Polyclonal Antibody, Unconjugated (bs-4720R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Molt-4 cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GDF9 Polyclonal Antibody(bs-4720R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).