bs-4727R
[Primary Antibody]
MRC1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MRC1
Immunogen Range: 201-300/1456
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4360
Swiss Prot: P22897
Source: KLH conjugated synthetic peptide derived from human MRC1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)
Predicted Molecular Weight: 160
Cross Reactive Species:
Human
Mouse
Rat
Dog
Predicted Cross Reactive Species:
Cow
Sheep
Pig
Horse
Chicken
Guinea Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Karmouty-Quintana, Harry, et al. "Deletion of ADORA2B from myeloid cells dampens lung fibrosis and pulmonary hypertension." The FASEB Journal (2014): fj-14.Read more>>
- Das, Anusuya, et al. "Bioactive lipid coating of bone allografts directs engraftment and fate determination of bone marrow-derived cells in rat GFP chimeras." Biomaterials (2015).Read more>>
- Azad, Abul K., et al. "γ-Tilmanocept, a New Radiopharmaceutical Tracer for Cancer Sentinel Lymph Nodes, Binds to the Mannose Receptor (CD206)." The Journal of Immunology (2015): 1402005.Read more>>
- Locatelli, Luigi, et al. "Macrophage recruitment by fibrocystin‐defective biliary epithelial cells promotes portal fibrosis in congenital hepatic fibrosis."Hepatology (2016).Read more>>
- Struecker, B., et al. "Implantation of a Tissue-Engineered Neo-Bile Duct in Domestic Pigs." European Surgical Research 56.1-2 (2015): 61-75.Read more>>
- Zhu, Dan, et al. "M2 Macrophage Transplantation Ameliorates Cognitive Dysfunction in Amyloid-β-Treated Rats Through Regulation of Microglial Polarization." Journal of Alzheimers Disease: 1-13.Read more>>
- Blomgran, Parmis, et al. "A possible link between loading, inflammation and healing: Immune cell populations during tendon healing in the rat." Scientific Reports 6 (2016): 29824.Read more>>
- Nakai et al. IL-17A induces heterogeneous macrophages, and it does not alter the effects of lipopolysaccharides on macrophage activation in the skin of mice. (2017) Sci.Rep. 7:12473Read more>>
- Nishikawa,et al.Highly Dispersible and Bioavailable Curcumin but not Native Curcumin Induces Brown-Like Adipocyte Formation in Mice.(2018) Molecular Nutrition & Food Research. 62:.Read more>>
- White,et al.GLUT12 promotes prostate cancer cell growth and is regulated by androgens and CaMKK2 signaling.(2018) Endocrine-Related Cancer. 25:453-469.Read more>>
- Shi F et al. Heat injured stromal cells-derived exosomal EGFR enhances prostatic wound healing afterthulium laser resection through EMT and NF-κB signaling. Prostate. 2019 May 24. Read more>>
- Ma R et al. Exploring the interactions between engineered nanomaterials and immune cells at 3D Nano-bio interfaces to discover potent Nano-adjuvants. Nanomedicine. 2019 Jun 18;21:102037. Read more>>
- Xie W et al. Dose Dependent Modulation of Bioactive Glasses Particles on Macrophage and Diabetic Wound Healing. Journal of Materials Chemistry B. 2018.Read more>>
- Žaloudíková M et al. The effect of exposure to hypoxia on superoxide formation by alveolar macrophages is indirect. Life Sci. 2019 Sep 10;236:116864.Read more>>
- Nishikawa S et al. Co-Administration of Curcumin and Artepillin C Induces Development of Brown-Like Adipocytes in Association with Local Norepinephrine Production by Alternatively Activated Macrophages in Mice. J Nutr Sci Vitaminol (Tokyo). 2019;65(4):328-334. Read more>>
- Shao N et al. A Multi‐Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy. Macromol Biosci. 2019 Nov 10:e1900255. Read more>>
- Jiang L et al. Sonodynamic Therapy in Atherosclerosis by Curcumin Nanosuspensions: Preparation design, Efficacy Evaluation, and Mechanisms Analysis. Eur J Pharm Biopharm. 2020 Jan;146:101-110.Read more>>
- Zhan Q et al. Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata Michx fruit. Int J Biol Macromol. 2019 Nov 12. pii: S0141-8130(19)37108-9.Read more>>
- Liu J et al. Polychlorinated biphenyl quinone promotes macrophage polarization to CD163+ cells through Nrf2 signaling pathway. Environmental Pollution,2019, 113587. Read more>>
- Shao N et al. A Multi‐Functional Silicon Nanoparticle Designed for Enhanced Osteoblast Calcification and Related Combination Therapy. Macromol Biosci. 2019 Nov 10:e1900255. Read more>>
- Liu S et al. Absorbable Thioether Grafted Hyaluronic Acid Nanofibrous Hydrogel for Synergistic Modulation of Inflammation Microenvironment to Accelerate Chronic Diabetic Wound Healing. Adv Healthc Mater. 2020 Apr 27:e2000198.Read more>>
- Tong?L et al. ACT001 reduces the expression of PD-L1 by inhibiting the phosphorylation of STAT3 in glioblastoma. Theranostics. 2020 May 1;10(13):5943-5956.Read more>>
- Gitto SB et al. Identification of a novel IL-5 signaling pathway in chronic pancreatitis and crosstalk with pancreatic tumor cells. Cell Commun Signal. 2020 Jun 17;18(1):95.Read more>>
- Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28Read more>>
- Hao Liu. et al. A 14 bp indel polymorphism in the promoter region is associated with different responses to porcine circovirus type 2 infection by regulating MRC1 transcription. Vet Immunol Immunop. 2021 Apr;234:110202Read more>>
- Sha Liu. et al. Dual Cross-linked HHA Hydrogel Supplies and Regulates M2 for Synergistic Improvement of Immunocompromise and Impaired Angiogenesis to Enhance Diabetic Chronic Wound Healing. Biomacromolecules. 2020;21(9):3795C3806Read more>>
- Jiyuan Piao. et al. Substance P blocks -aminopropionitrile-induced aortic injury through modulation of M2 monocyte-skewed monocytopoiesis. Transl Res. 2021 Feb;228:76Read more>>
- Xiaowei Li. et al. Pristane promotes anaerobic glycolysis to facilitate proinflammatory activation of macrophages and development of arthritis. Exp Cell Res. 2021 Jan;398:112404Read more>>
- Simon Harper. et al. Portal venous repopulation of decellularised rat liver scaffolds with syngeneic bone marrow stem cells. J Tissue Eng Regen M. 2020 Oct;14(10):1502-1512Read more>>
- Kohei Kamada. et al. Attenuation of Knee Osteoarthritis Progression in Mice through Polarization of M2 Macrophages by Intra-Articular Transplantation of Non-Cultured Human Adipose-Derived Regenerative Cells. J Clin Med. 2021 Jan;10(19):4309Read more>>
- Meng Lin. et al. CRISPR-based in situ engineering tumor cells to reprogram macrophages for effective cancer immunotherapy. Nano Today. 2022 Feb;42:101359Read more>>
- Nguyen, Thao K. T.. et al. Recombinant Human Lactoferrin Reduces Inflammation and Increases Fluoroquinolone Penetration to Primary Granulomas During Mycobacterial Infection of C57Bl/6 Mice. Arch Immunol Ther Ex. 2022 Dec;70(1):1-13Read more>>
- Jing Wang. et al. Mesenchymal stem cells regulate M1 polarization of peritoneal macrophages through the CARD9-NF-_B signaling pathway in severe acute pancreatitis. J HEPATO-BIL-PAN SCI. 2022 Jun 23Read more>>
- Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683Read more>>
- Linghao Xiao. et al. Osteogenic capacity of octacalcium phosphate involving macrophage polarization. J BIOMED MATER RES A. 2022 DecRead more>>
- Xin Li. et al. Paeonol alleviates neuropathic pain by modulating microglial M1 and M2 polarization via the RhoA/p38MAPK signaling pathway. CNS NEUROSCI THER. 2023 ApRead more>>
- Kagioka Takumi. et al. Lithium carbonate accelerates the healing of apical periodontitis. SCI REP-UK. 2023 May;13(1):1-12Read more>>
- Wu Guanghao. et al. Thylakoid engineered M2 macrophage for sonodynamic effect promoted cell therapy of early atherosclerosis. NANO RES. 2023 Sep;:1-1Read more>>
- Yanan Jiang. et al. A MetalCOrganic Framework-Incorporated Hydrogel for Delivery of Immunomodulatory Neobavaisoflavone to Promote Cartilage Regeneration in Osteoarthritis. ACS APPL MATER INTER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Tohru Mutoh. et al. Spatiotemporal expression patterns of ZBP1 in the brain of mouse experimental stroke model. J CHEM NEUROANAT. 2023 Dec;134:102362Read more>>
VALIDATION IMAGES
HepG2 cells(black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Antibody（bs-4727R） at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MRC1 Polyclonal Antibody(bs-4727R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).