bs-4755R [Primary Antibody]
CD19 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD19

Immunogen Range: 151-250/556


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 930

Swiss Prot: P15391

Source: KLH conjugated synthetic peptide derived from human CD19

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 59


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Pig
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiong Y et al. Functions of T-cell subsets and their related cytokines in the pathological processes of autoimmune encephalomyelitic mice.(2018) Int J Clin Exp Pathol;11(10):4817-4826.Read more>>
  • Sahiner et al. Impact of intravesical hyaluronic acid treatment on bladder inflammation in interstitial cystitis rat model. (2018) Int.Braz.J.Urol. 44:1014-1022Read more>>
  • oon Ching Teeet al. Xenogeneic mesenchymal stem cell transplantation for mandibular defect regeneration. Xenotransplantation. 2020 Sep;27(5):e12625.Read more>>
  • T. Shikayama. et al. Hematogenous apoptotic mechanism in salivary glands in chronic periodontitis. Arch Oral Biol. 2020 Sep;117:104775Read more>>
VALIDATION IMAGES

Mouse lung and liver lysates probed with CD19 Polyclonal Antibody, unconjugated (bs-4755R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.\n


Human HL60 Cells were fixed with 70% methanol (Overnight at 4℃). The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by incubation with CD19 Polyclonal Antibody, Unconjugated (bs-4755R) at 0.2 μg/10^6 cells for 30 minutes at room temp. A PE-conjugated secondary antibody was used for 40 min at room temperature. The graph compares the primary antibody (green) to unstained cells (dark blue), secondary only (light blue), and isotype control (orange).