bs-4790R-FITC [Conjugated Primary Antibody]
CD8 Polyclonal Antibody, FITC Conjugated
www.biossusa.com
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DATASHEET

Host: Rabbit

Target Protein: CD8

Immunogen Range: 51-150/235


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 925

Swiss Prot: P01732

Source: KLH conjugated synthetic peptide derived from human CD8

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 27


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zou, Ming-Xiang, et al. "Clinicopathologic implications of CD8+/Foxp3+ ratio and miR-574-3p/PD-L1 axis in spinal chordoma patients." Cancer Immunology, Immunotherapy (2017): 1-16.Read more>>
  • Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
  • Zou MX et al. Clinical Impact of the Immune Microenvironment in Spinal Chordoma: Immunoscore as an Independent Favorable Prognostic Factor.Neurosurgery. 2018 Jul 19. Read more>>
  • Wu Yutong. et al. Osteoclast-Derived Apoptotic Bodies Inhibit Naive Cd8<sup> </sup> T Cell Activation via Siglec15 Promoting Breast Cancer Secondary Metastasis. Cell Reports Medicine. 2022 Nov 03Read more>>
  • Jia-qi Yuan. et al. S100A9 promotes glycolytic activity in HER2-positive breast cancer to induce immunosuppression in the tumour microenvironment. HELIYON. 2023 Feb;9:e13294Read more>>
  • Lee Yi-Mei. et al. Genomic and Transcriptomic Landscape of an Oral Squamous Cell Carcinoma Mouse Model for Immunotherapy. CANCER IMMUNOL RES. 2023 SepRead more>>
  • Misato Horie. et al. Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a premetastatic microenvironment. MOL ONCOL. 2023 SepRead more>>
VALIDATION IMAGES

Molt-4 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD8 Polyclonal Antibody(bs-4790R-FITC)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).