Host: Rabbit
Target Protein: CD8 alpha
Immunogen Range: 51-150/236
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 24930
Swiss Prot: P07725
Source: KLH conjugated synthetic peptide derived from rat CD8 alpha
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 27
Cross Reactive Species:
Human
Rat
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Cheng, Lin, et al. "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." Diagnostic Pathology 8.1 (2013): 168.Read more>>
- Savvatis, Konstantinos, et al. "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." Basic Research in Cardiology 109.6 (2014): 1-14.Read more>>
- Hu X et al. Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus. Chemosphere. 2019 Jul 22;237:124427. Read more>>
Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C and DAPI staining.
Lane 1: Rat Thymus lysates; Lane 2: Rat lymph node lysates probed with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.