bs-4791R [Primary Antibody]
CD8 alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD8 alpha

Immunogen Range: 60-110/236

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 24930

Swiss Prot: P07725

Source: KLH conjugated synthetic peptide derived from rat CD8 alpha

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cheng, Lin, et al. "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." Diagnostic Pathology 8.1 (2013): 168.Read more>>
  • Savvatis, Konstantinos, et al. "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." Basic Research in Cardiology 109.6 (2014): 1-14.Read more>>
  • Hu X et al. Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus. Chemosphere. 2019 Jul 22;237:124427. Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (bs-0295G-Cy3) used at 1:200 dilution for 40 minutes at 37°C. DAPI was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Paraformaldehyde-fixed, paraffin embedded rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:200 overnight at 4°C, followed by a conjugated secondary Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C and DAPI staining.


Lane 1: Rat Thymus lysates; Lane 2: Rat lymph node lysates probed with CD8 alpha Polyclonal Antibody, Unconjugated (bs-4791R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.