bs-4815R [Primary Antibody]
CD3E Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD3E

Immunogen Range: 23-120/189


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12501

Swiss Prot: P22646

Source: KLH conjugated synthetic peptide derived from mouse CD3 epsilon

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The CD3 complex mediates signal transduction.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IF()

Predicted Molecular Weight: 20


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chenna Narendra S, Chalise JP, Magnusson M, Uppugunduri S (2015) Local but Not Systemic Administration of Uridine Prevents Development of Antigen-Induced Arthritis. PLoS ONE 10(10): e0141863Read more>>
  • Da?Won Choi. et al. Co?transplantation of tonsil?derived mesenchymal stromal cells in bone marrow transplantation promotes thymus regeneration and T cell diversity following cytotoxic conditioning. Int J Mol Med. 2020 Sep;46(3):1166-1174Read more>>
  • Quanyu Chen. et al. Comparative effects of hepatocyte growth factor and tacrolimus on acute liver allograft early tolerance. FRONT IMMUNOL. 2023; 14: 1162439Read more>>
VALIDATION IMAGES

Mouse spleen lysate probed with Anti-CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at 37˚C.


Lane 1: Human MOLT4 cell lysates; Lane 2: Human Jurkat cell lysates probed with CD3E Polyclonal Antibody, Unconjugated (bs-4815R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.