bs-4834R [Primary Antibody]
CD73 Polyclonal Antibody
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Host: Rabbit

Target Protein: CD73

Immunogen Range: 151-250/574


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4907

Swiss Prot: P21589

Source: KLH conjugated synthetic peptide derived from human CD73/NT5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 63


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Pig
Rabbit
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gao, Yuhua, et al. "Isolation and Characterization of Chicken Dermis-Derived Mesenchymal Stem/Progenitor Cells." BioMed Research International 2013 (2013).Read more>>
  • Long, Qianfa, et al. "Genetically engineered bone marrow mesenchymal stem cells improve functional outcome in a rat model of epilepsy." Brain Research (2013).Read more>>
  • Takemitsu, Hiroshi, et al. "Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells." BMC Veterinary Research 8.1 (2012): 150.Read more>>
  • Lu, Taofeng, et al. "Isolation and Characterization of Adipose-derived Mesenchymal Stem Cells (ADSCs) from Cattle." Applied Biochemistry and Biotechnology (2014): 1-10.Read more>>
  • Gao, Yuhua, et al. "All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells." Applied Biochemistry and Biotechnology (2014): 1-11.Read more>>
  • Gao, Yuhua, et al. "All-trans Retinoic Acid Promotes Nerve Cell Differentiation of Yolk Sac-Derived Mesenchymal Stem Cells." Applied Biochemistry and Biotechnology (2014): 1-11.Read more>>
  • Long, Qianfa, et al. "MRI tracking bone marrow mesenchymal stem cells labeled with ultra-small superparamagnetic iron oxide nanoparticles in a rat model of temporal lobe epilepsy." Neuroscience Letters (2015).Read more>>
  • Wang, Chenghe, et al. "Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD." Official Journal of the Brazilian Society of Urology 41.4 (2015): 764-772.Read more>>
  • Long, Q., et al. "Bone marrow mesenchymal stem cell transplantation improves cognitive impairment via up-regulation of hippocampal GABAergic system in a rat model of chronic cerebral hypoperfusion." Neuroscience (2015).Read more>>
  • Chen, Jia, et al. "Biological characterization of metanephric mesenchymal stem cells from the Beijing duck." Experimental and Therapeutic Medicine 11.2 (2016): 439-447.Read more>>
  • Lin, Hsing-Yi, et al. "Isolation and characterization of multipotent mesenchymal stem cells adhering to adipocytes in canine bone marrow." Stem Cells and Development ja (2016).Read more>>
  • Ivanovska, Ana, et al. "Immunophenotypical characterization of canine mesenchymal stem cells from perivisceral and subcutaneous adipose tissue by a species-specific panel of antibodies." Research in Veterinary Science (2017).Read more>>
  • Ma, Caiyun, et al. "Cryopreservation and multipotential characteristics evaluation of a novel type of mesenchymal stem cells derived from Small Tailed Han Sheep fetal lung tissue." Cryobiology (2017).Read more>>
  • Gao et al. Common expression of stemness molecular markers and early cardiac transcription factors in human Wharton's jelly-derived mesenchymal stem cells and embryonic stem cells. (2013) Cell.Transplan. 22:1883-900Read more>>
  • Long et al. Mash1-dependent Notch Signaling Pathway Regulates GABAergic Neuron-Like Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells. (2017) Aging.Di. 8:301-313Read more>>
  • Li R et al. Metabolomic analysis reveals metabolic alterations of human peripheral blood lymphocytes by perfluorooctanoic acid. Chemosphere. 2019 Sep 7;239:124810. Read more>>
  • Chen F et al. The biological characteristics of sheep umbilical cord mesenchymal stem cells.Can J Vet Res. 2018 Jul;82(3):216-224.Read more>>
  • Lu T et al. In vitro culture and biological properties of broiler adipose?derived stem cells.Exp Ther Med. 2018 Sep;16(3):2399-2407.Read more>>
  • Chen F et al. The biological characteristics of sheep umbilical cord mesenchymal stem cells.Can J Vet Res. 2018 Jul;82(3):216-224.Read more>>
  • Lu T et al. In vitro culture and biological properties of broiler adipose?derived stem cells.Exp Ther Med. 2018 Sep;16(3):2399-2407.Read more>>
  • Mei X et al. Expression of VEGF, CD73 and their relationship with clinical pathology, microvessel density, and prognosis in renal cell carcinoma. Transl Androl Urol. 2020 Jun;9(3):1366-1373.Read more>>
  • Gae Won Parket al. Topical cell-free conditioned media harvested from adipose tissue-derived stem cells promote recovery from corneal epithelial defects caused by chemical burns. Sci Rep. 2020 Jul 24;10(1):12448.Read more>>
  • Jingchuan Sun. et al. Long noncoding RNA AC092155 facilitates osteogenic differentiation of adipose-derived stem cells through the miR-143-3p/STMN1 axis. 2021 May 15Read more>>
  • Wei-Cheng Tseng. et al. Hypoxic mesenchymal stem cells ameliorate acute kidney ischemia-reperfusion injury via enhancing renal tubular autophagy. Stem Cell Res Ther. 2021 Dec;12(1):1-22Read more>>
  • Jianyun Cao. et al. Puerarin promotes the osteogenic differentiation of rat dental follicle cells by promoting the activation of the nitric oxide pathway. Tissue Cell. 2021 Dec;73:101601Read more>>
  • Nina Kre?i?. et al. The Expression Pattern of Surface Markers in Canine Adipose-Derived Mesenchymal Stem Cells. Int J Mol Sci. 2021 Jan;22(14):7476Read more>>
  • Chi Young Chang. et al. Novel method to repair articular cartilage by direct reprograming of prechondrogenic mesenchymal stem cells. Eur J Pharmacol. 2021 Nov;911:174416Read more>>
  • Ruijing Zhao. et al. Inhalation of MSC-EVs is a noninvasive strategy for ameliorating acute lung injury. J Control Release. 2022 May;345:214Read more>>
  • Jinsheng Li. et al. Micro/nano-topography Promotes Osteogenic Differentiation of Bone Marrow Stem Cells by Regulating Periostin Expression. COLLOID SURFACE B. 2022 Jul;:1127Read more>>
  • Minji Choi. et al. Effect of Co-culture of mesenchymal stem cell and glomerulus endothelial cell to promote endothelialization under optimized perfusion flow rate in whole renal ECM scaffold. MATER TODAY BIO. 2022 Dec;17:100464Read more>>
  • Yinhuan Zhang. et al. Discovery of Hepatotoxic Equivalent Markers and Mechanism of Polygonum multiflorum Thunb. by Metabolomics Coupled with Molecular Docking. MOLECULES. 2023 Jan;28(1):25Read more>>
  • Emma Heyman. et al. Validation of multiparametric panels for bovine mesenchymal stromal cell phenotyping. CYTOM PART A. 2023 MayRead more>>
  • Jiangang Han. et al. Transcriptome profiling of differentiating adipose-derived stem cells across species reveals new genes regulating adipogenesis. BBA-MOL CELL BIOL L. 2023 Aug;:159378Read more>>
  • Yue Wu. et al. The biological characteristics of chicken embryo mesenchymal stem cells isolated from chorioallantoic membrane. GENESIS. 2024 Apr;62(2):e23592Read more>>
  • Jie Yang. et al. Neohesperidin alleviates the inhibitory effect of bisphenol A on the myogenic differentiation of umbilical cord mesenchymal stem cells via the IGF1R/AKT1/RHOA signaling pathway. ECOTOX ENVIRON SAFE. 2024 Sep;283:116804Read more>>
  • Ze Zhang. et al. DKK3 promotes adipogenic differentiation of stem cells by inhibiting Wnt/-catenin signaling pathway related gene expression and mitochondrial autophagy function. POULTRY SCI. 2024 Aug;:104257Read more>>
VALIDATION IMAGES

Mouse kidney lysates probed with CD73 Polyclonal Antibody, unconjugated (bs-4834R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.


The U-87MG (H) cells were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.) , followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green): Rabbit Anti-CD73 antibody (bs-4834R): 1 μg/10^6 cells; Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.