VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-Complement fragment 3c Antibody, conjugated (bs-4877R-FITC) at 1:400 overnight at 4°C; DAPI was used to stain the cell nuclei
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-Complement fragment 3c Antibody, conjugated (bs-4877R-CY3) at 1:400 overnight at 4°C; DAPI was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.
Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:100 overnight at 4°C, followed by secondary and DAB staining.
Lane 1: Human HepG2 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A549 cell lysates probed with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-42105R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human HepG2 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A549 cell lysates probed with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.