bs-4877R [Primary Antibody]
Complement C3d fragment Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Complement C3d fragment

Immunogen Range: 1002-1100/1663


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 718

Swiss Prot: P01024

Source: KLH conjugated synthetic peptide derived from human Complement C3d fragment

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 33/109/181


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-Complement fragment 3c Antibody, conjugated (bs-4877R-FITC) at 1:400 overnight at 4°C; DAPI was used to stain the cell nuclei


Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation: Anti-Complement fragment 3c Antibody, conjugated (bs-4877R-CY3) at 1:400 overnight at 4°C; DAPI was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human rectal cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Human lung cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-FITC) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:100 overnight at 4°C, followed by secondary and DAB staining.


Lane 1: Human HepG2 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A549 cell lysates probed with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-42105R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human HepG2 cell lysates; Lane 2: Human U87MG cell lysates; Lane 3: Human A431 cell lysates; Lane 4: Human HeLa cell lysates; Lane 5: Human A549 cell lysates probed with Complement C3d fragment Polyclonal Antibody, Unconjugated (bs-4877R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.