bs-4890R [Primary Antibody]
CD24 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD24

Immunogen Range: 27-59/80


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 100133941

Swiss Prot: P25063

Source: KLH conjugated synthetic peptide derived from human CD24

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)

Predicted Molecular Weight: 8


Cross Reactive Species: Human
Mouse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Human A549 cells probed with CD24 Polyclonal Antibody (bs-4890R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG, FITC Conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue) and secondary only (light blue)


A431 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD24 Polyclonal Antibody (bs-4890R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).