DATASHEET
Host:
Rabbit
Target Protein:
CD24
Immunogen Range:
27-59/80
Clonality:
Polyclonal
Isotype:
IgG
Entrez Gene:
100133941
Swiss Prot:
P25063
Source:
KLH conjugated synthetic peptide derived from human CD24
Purification:
Purified by Protein A.
Storage Buffer:
0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage:
Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells.
VALIDATION IMAGES
Human A549 cells probed with CD24 Polyclonal Antibody (bs-4890R) at 1:50 for 40 minutes followed by incubation with Goat Anti-Rabbit IgG, FITC Conjugated secondary (bs-0295G-FITC) at 1:100 (green) for 40 minutes compared to control cells (blue) and secondary only (light blue)
A431 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CD24 Polyclonal Antibody (bs-4890R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).