bs-4891R [Primary Antibody]
CD24 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD24

Immunogen Range: 27-53/76


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12484

Swiss Prot: P24807

Source: KLH conjugated synthetic peptide derived from mouse CD24

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May have a specific role to play in early thymocyte development.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 8


Cross Reactive Species: Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Sinkemani A et al. Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic ConditionsStem Cells Int.2020 Dec 23;2020:8882549.Read more>>
VALIDATION IMAGES

U-937 cells were stained with CD24 Polyclonal Antibody(bs-4891R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Mouse Spleen tissue lysates; Lane 2: Mouse Bone tissue lysates; Lane 3: Mouse Kidney tissue lysates; Lane 4: Mouse Cerebrum tissue lysates probed with CD24 Polyclonal Antibody, Unconjugated (bs-4891R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.