bs-4902R [Primary Antibody]
Calponin 2 + 3 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Calponin 2 + 3

Immunogen Range: 101-200/309

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1264

Swiss Prot: P51911

Source: KLH conjugated synthetic peptide derived from human Calponin 2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months..

Background: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
IEM(1:20-200)
IGS(1:20-200)
GICA(1:20-200)

Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lovren, Fina, et al. "MicroRNA-145 Targeted Therapy Reduces Atherosclerosis." Circulation 126.11 suppl 1 (2012): S81-S90.Read more>>
VALIDATION IMAGES

cells probed with Rabbit Anti-Calponin 2 + 3 Polyclonal Antibody (bs-4902R) at 1:100 for 30 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary at 1:100 for 30 minutes (green) compared to control cells (blue), secondary only (light blue) and isotype control (orange)


Paraformaldehyde-fixed, paraffin embedded rat heart; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Calponin 2 + 3 Polyclonal Antibody, Unconjugated (bs-4902R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining