bs-4913R [Primary Antibody]
ENPP1 Polyclonal Antibody
www.biossusa.com
[email protected]
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+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: ENPP1

Immunogen Range: 41-140/925


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5167

Swiss Prot: P22413

Source: KLH conjugated synthetic peptide derived from human ENPP1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

By generating PPi, plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. PPi inhibits mineralization by binding to nascent hydroxyapatite (HA) crystals, thereby preventing further growth of these crystals. Preferentially hydrolyzes ATP, but can also hydrolyze other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity and function.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 100


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang Qiang. et al. ENPP1 deletion causes mouse osteoporosis via the MKK3/p38 MAPK/PCNA signaling pathway. J ORTHOP SURG RES. 2022 Dec;17(1):1-9Read more>>
  • Gao Zhiqiang. et al. Enpp1 deficiency caused chondrocyte apoptosis by inhibiting AMPK signaling pathway. J ORTHOP SURG RES. 2023 Dec;18(1):1-1Read more>>
VALIDATION IMAGES

Lane 1: Human HepG2 cell lysates; Lane 2: Human SH-SY5Y cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human Huvec cell lysates ; Lane 5: Human MDA-MB-231 cell lysates probed with ENPP1 Polyclonal Antibody, Unconjugated (bs-4913R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.