bs-4917R [Primary Antibody]
Osteocalcin Polyclonal Antibody
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Host: Rabbit

Target Protein: Osteocalcin

Immunogen Range: 21-100/100


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 632

Swiss Prot: P02818

Source: KLH conjugated synthetic peptide derived from human Osteocalcin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Osteocalcin belongs to the osteocalcin/matrix Gla protein family and constitutes 1 to 2% of the total bone protein. It is a 49 amino acid single chain vitamin K dependent protein, made by osteoblasts, and is a major component of the noncollagenous bone matrix. Post translational modification by a vitamin K dependent carboxylase produces three gamma carboxyglutamic acid residues at positions 17, 21 and 24, giving it a high affinity for calcium. It also binds strongly to apatite.

Size: 100µL

Concentration: 1ug/ul

Predicted Molecular Weight: 11


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Ding, Shan, et al. "A nano-micro alternating multilayer scaffold loading with rBMSCs and BMP-2 for bone tissue engineering." Colloids and Surfaces B: Biointerfaces (2015).Read more>>
  • Bugueño, Juan, et al. "Canine mesenchymal stem cell bone regenerative capacity is regulated by site-specific multi-lineage differentiation." Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology (2016).Read more>>
  • Jiang et al. Andrographolide Exerts Pro-Osteogenic Effect by Activation of Wnt/β-Catenin Signaling Pathway in Vitro. (2015) Cell.Physiol.Bioche. 36:2327-39Read more>>
  • Liu et al. MicroRNA-155 inhibits the osteogenic differentiation of mesenchymal stem cells induced by BMP9 via downregulation of BMP signaling pathway. (2018) Int.J.Mol.Med. 41:3379-3393Read more>>
  • Zhang Y et al. Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness. (2018) Biomed Res Int. Nov 1;2018:4025083.Read more>>
  • Kim et al. Membrane-Based Microwave-Mediated Electrochemical Immunoassay for the In Vitro, Highly Sensitive Detection of Osteoporosis-Related Biomarkers. (2018) Sensors.(Basel).Sep 4;18(9). Read more>>
  • Liu G et al. Suppressing MicroRNA-30b by Estrogen Promotes Osteogenesis in Bone Marrow MesenchymalStem Cells. Stem Cells Int. 2019 Apr 4;2019:7547506.Read more>>
  • Nakamura S et al. Acceleration of Bone Regeneration of Horizontal Bone Defect in Rats Using Collagen‐Binding Basic Fibroblast Growth Factor Combined with Collagen Scaffolds.J Periodontol. 2019 Mar 19. Read more>>
  • Xiao Y et al. Controlled delivery of recombinant human bone morphogenetic protein-2 by using glucose-sensitive core–shell nanofibers to repair the mandible defects in diabetic rats. J. Mater. Chem. B, 2019,7, 4347-4360. Read more>>
  • Sun L et al. MiR-26a promotes fracture healing of nonunion rats possibly by targeting SOSTDC1 and further activating Wnt/β-catenin signaling pathway. Mol Cell Biochem. 2019 Jul 16.Read more>>
  • Fan C et al. Cross-linked gelatin microsphere-based scaffolds as a delivery vehicle of MC3T3-E1 cells: in vitro and in vivo evaluation. Materials Science and Engineering: C,2019 110399. Read more>>
  • Zhu H et al. Direct reprogramming of mouse fibroblasts into functional osteoblasts. J Bone Miner Res. 2019 Dec 2.Read more>>
  • Ginini JG et al. Effects of Timing of Extracorporeal Shock Wave Therapy on Mandibular Distraction Osteogenesis: An Experimental Study in a Rat Model.J Oral Maxillofac Surg. 2018 Jul 24. pii: S0278-2391(18)30838-3. Read more>>
  • Liu Y et al. The synergistic effect of NELL1 and adipose-derived stem cells on promoting bone formation in osteogenesis imperfecta treatment. Biomed Pharmacother. 2020 Aug;128:110235.Read more>>
  • Liu M et al. Luminescent Net-like Inorganic Scaffolds with Europium Doped Hydroxyapatite for Enhanced Bone ReconstructionNanoscale.2021 Jan 6.Read more>>
  • Ramesh Subbiah. et al. Prevascularized hydrogels with mature vascular networks promote the regeneration of critical\size calvarial bone defects in vivo. 2021 Feb 05Read more>>
  • Zheng-Zhao Liu . et al. Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. 2020 Nov 04Read more>>
  • Qiuping Leng. et al. Demineralized bone matrix scaffold modified with mRNA derived from osteogenically pre-differentiated MSCs improves bone repair. Mat Sci Eng C-Mater. 2021 Feb;119:111601Read more>>
  • Zhang Chen. et al. The Histone Demethylase KDM3B Promotes Osteo-/Odontogenic Differentiation, Cell Proliferation, and Migration Potential of Stem Cells from the Apical Papilla. Stem Cells Int. 2020;2020:8881021Read more>>
  • Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084Read more>>
  • Komei Kawamoto. et al. A Study of Bone Formation around Titanium Implants Using Frozen Sections. 2021 Apr 15Read more>>
  • Yi Liu. et al. Modification of COL1A1 in Autologous Adipose Tissue-Derived Progenitor Cells Rescues the Bone Phenotype in a Mouse Model of Osteogenesis Imperfecta. 2021 May 18Read more>>
  • Matja? Fin?gar. et al. The development and characterization of bioactive coatings for local drug delivery in orthopedic applications. Prog Org Coat. 2021 Sep;158:10635Read more>>
  • Fangyu Qiao. et al. 4-Octyl itaconate modified demineralized bone matrix scaffold improves bone repair by regulating early inflammation. Chem Eng J. 2021 Dec;425:13149Read more>>
  • Zhang, Zilong. et al. Circ_FBLN1 promotes the proliferation and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells by regulating let-7i-5p/FZD4 axis and Wnt/-catenin pathway. 2021 Aug 23Read more>>
  • Antoine Karoichan. et al. Encapsulation and differentiation of adipose-derived mesenchymal stem cells in a biomimetic purine cross-linked chitosan sponge. 2021 Sep 21Read more>>
  • Zheng, Han. et al. miR-140-3p enhanced the osteo/odontogenic differentiation of DPSCs via inhibiting KMT5B under hypoxia condition. Int J Oral Sci. 2021 Dec;13(1):1-1Read more>>
  • Qingnan Yan. et al. Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling. J Biol Chem. 2022 Jan;:101639Read more>>
  • Samantha Ketelyn Silva. et al. Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation. INT J MOL SCI. 2022 Jan;23(12):6503Read more>>
  • Fang Dai. et al. Effects of long term diabetogenic high fat diet on bone in ovariectomized female rats. BIOTECH HISTOCHEM. 2022 Jun 28Read more>>
  • Maximilian Koblenzer. et al. Physiological Mineralization during In Vitro Osteogenesis in a Biomimetic Spheroid Culture Model. CELLS-BASEL. 2022 Jan;11(17):2702Read more>>
  • Ye Zhang. et al. Effect of Hydroxyapatite Coating by Er: YAG Pulsed Laser Deposition on the Bone Formation Efficacy by Polycaprolactone Porous Scaffold. INT J MOL SCI. 2022 Jan;23(16):9048Read more>>
  • Du Zhi. et al. Circulating exosomal circRNA_0063476 impairs expression of markers of bone growth via the miR-518c-3p/DDX6 axis in ISS. ENDOCRINOLOGY. 2022 AugRead more>>
  • Katarzyna Klimek. et al. Could Curdlan/Whey Protein Isolate/Hydroxyapatite Biomaterials Be Considered as Promising Bone Scaffolds?—Fabrication, Characterization, and Evaluation of Cytocompatibility towards Osteoblast Cells In Vitro. CELLS-BASEL. 2022 Jan;11(20):3251Read more>>
  • Guangyue Li. et al. Irisin Promotes Osteogenesis by Modulating Oxidative Stress and Mitophagy through SIRT3 Signaling under Diabetic Conditions. OXID MED CELL LONGEV. 2022 Oct 10;2022:3319056Read more>>
  • Dan Li. et al. A HAase/NIR responsive surface on titanium implants for treating bacterial infection and improving osseointegration. J MATER SCI TECHNOL. 2022 NovRead more>>
  • ?brahim Halilullah Erbay. et al. Bioengineering bone-on-a-chip model harnessing osteoblastic and osteoclastic resolution. ADV ENG MATER. 2022 DecRead more>>
  • Lin, Xiaoxin. et al. Shikonin promotes rat periodontal bone defect repair and osteogenic differentiation of BMSCs by p38 MAPK pathway. ODONTOLOGY. 2022 Dec;:1-9Read more>>
  • Yadong Yang. et al. Effects of highly selective sensory/motor nerve injury on bone metabolism and bone remodeling in rats.. J MUSCULOSKEL NEURON. 2022 Dec;22(4):524-535Read more>>
  • Jinxiu Yu. et al. Promoting osseointegration of titanium by pH-responsive releasing of H2S and optimizing polarization time for macrophages. COMPOS PART B-ENG. 2023 Mar;253:110554Read more>>
  • Yixuan Li. et al. Minimally invasive bone augmentation through subperiosteal injectable hydroxylapatite/laponite/alginate nanocomposite hydrogels. INT J BIOL MACROMOL. 2023 Mar;231:123232Read more>>
  • Nan Wang. et al. Histological analysis of pulp mineralisation after severe intrusive luxation of immature molars in rats. DENT TRAUMATOL. 2023 FebRead more>>
  • Kou Yuying. et al. Eldecalcitol prevented OVX-induced osteoporosis through inhibiting BMSCs senescence by regulating the SIRT1-Nrf2 signal. FRONT PHARMACOL. 2023 Mar;14:504Read more>>
  • Zhenyin Chen. et al. Dynamic stiffening collagen-coated substrate enhances osteogenic differentiation of mesenchymal stem cells through integrin 21. BIOMATER SCI-UK. 2023 MayRead more>>
  • Xiao Han. et al. METTL3 Promotes Osteo/Odontogenic Differentiation of Stem Cells by Inhibiting miR-196b-5p Maturation. STEM CELLS INT. 2023;2023:8992284Read more>>
  • Chenchen Yang. et al. Study on the mechanism of NLRP3 effect on the skeleton of de-ovalized mice. Biochemistry and Biophysics Reports. 2023 Sep;35:101496Read more>>
  • Hongrong Zhang. et al. Extracellular matrix of ameloblastoma-derived negatively regulates osteogenic differentiation. ORAL DIS. 2023 JulRead more>>
  • Tuo Shi. et al. The Synergistic Effect of Zuogui Pill and Eldecalcitol on Improving Bone Mass and Osteogenesis in Type 2 Diabetic Osteoporosis. MED LITH. 2023 Aug;59(8):1414Read more>>
  • Jia Song. et al. Manipulation of Surface Electrical Charge on Nanocomposite Membranes Confers Wide Spectrum Bactericidal Effects and Promotes Tissue Regeneration. ADV FUNCT MATER. 2024 Feb;:2314024Read more>>
  • Qingyun Fu. et al. Polydopamine-modified metal-organic frameworks nanoparticles enhance the corrosion resistance and bioactivity of polycaprolactone coating on high-purity magnesium. J MAGNES ALLOY. 2024 FebRead more>>
VALIDATION IMAGES

Human U2-OS cells were stained with Osteocalcin Polyclonal Antibody, Unconjugated(bs-4917R) at 1:200 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.


Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:100 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.


Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded (human skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (bs-4917R) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF488) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Testicles; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ovarian Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Osteocalcin polyclonal antibody, unconjugated (bs-4917R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Ewing’s sarcoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Osteocalcin Polyclonal Antibody, Unconjugated (bs-4917R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Osteocalcin polyclonal antibody, unconjugated (bs-4917R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.