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Host: Rabbit
Target Protein: EAAT2
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6506
Swiss Prot: P43006
Source: Recombinant mouse EAAT2 protein
Purification: Purified by Protein A.
Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Store at -20°C for 12 months.
Background:
Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate.
Functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. Mediates Cl- flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na+ symport.
Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate.
Conjugation: Biotin
Excitation/ Emission: N/A
Size: 100µL
Concentration: 1mg/ml
Applications:
WB(WB=1:500-2000)
IHC-P(IHC-P=1:400-800)
IHC-F(IHC-F=1:400-800)
IF(IF=1:100-500)
Predicted Molecular Weight: 62
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EAAT2 Polyclonal Antibody (bs-49183R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EAAT2 Polyclonal Antibody (bs-49183R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EAAT2 Polyclonal Antibody (bs-49183R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EAAT2 Polyclonal Antibody (bs-49183R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EAAT2 Polyclonal Antibody (bs-49183R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EAAT2 Polyclonal Antibody (bs-49183R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with EAAT2 Polyclonal Antibody (bs-49183R-Bio) at 1:200 overnight at 4°C, followed by conjugation to the Streptavidin-HRP（bs-0437P-HRP）and DAB (C-0010) staining.