bs-4938R-RBITC [Conjugated Primary Antibody]
CXCL12 Polyclonal Antibody, RBITC Conjugated
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Host: Rabbit

Target Protein: CXCL12

Immunogen Range: 24-70/93


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6387

Swiss Prot: P48061

Source: KLH conjugated synthetic peptide derived from human SDF-1/CXCL12

Purification: purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.

Conjugation: RBITC

Excitation/ Emission: 570nm/595nm

Size: 100ul

Concentration: 1mg/ml

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
IHC-P(1:100-500)
IF(1:100-500)

Predicted Molecular Weight: 8 kDa


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Chicken
Pig
Rabbit
GuineaPig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Pei, Guangchang, et al. "Renal Interstitial Infiltration and Tertiary Lymphoid Organ Neogenesis in IgA Nephropathy." Clinical Journal of the American Society of Nephrology (2013): CJN-01150113.Read more>>
  • Grootaert, Mandy OJ, et al. "Defective autophagy in vascular smooth muscle cells accelerates senescence and promotes neointima formation and atherogenesis." Autophagy (2015).Read more>>
  • Wang, Xiao-yan, et al. "AMD3100 attenuates MMP-3 and MMP-9 expressions and prevents cartilage degradation in a monosodium iodoacetate-induced rat model of temporomandibular osteoarthritis." Journal of Oral and Maxillofacial Surgery (2016).Read more>>
  • Qi D et al. Stromal cell\derived factor\1 regulates the secretion of interleukin\1 in the temporomandibular joint of rats with synovial inflammation. J Oral Pathol Med. 2020 Oct;49(9):933-939.Read more>>
  • Kawamura Teruhiko. et al. Identification of genes associated with endometrial cell ageing. Mol Hum Reprod. 2021 Feb;27(2)Read more>>
  • Yan-Cong Wang. et al. NRSF deficiency leads to abnormal postnatal development of dentate gyrus and impairment of progenitors in subgranular zone of hippocampus. 2021 May 07Read more>>
  • Jikai Wang. et al. MFGE8 mitigates brain injury in a rat model of SAH by maintaining vascular endothelial integrity via TIG5/PI3K/CXCL12 signaling. 2021 May 15Read more>>
  • Narubhorn Ongprakobkul. et al. Effects of local vs systemic administration of CXCR4 inhibitor AMD3100 on orthodontic tooth movement in rats. Am J Orthod Dentofac. 2022 MaRead more>>
  • Chun-Yi Ng. et al. Endothelial-immune crosstalk contributes to vasculopathy in nonalcoholic fatty liver disease. EMBO REP. 2022 Jun;23(6):e54271Read more>>
  • Lingjie Yang. et al. Screening of the best time window for MSC transplantation to treat acute myocardial infarction with SDF-1 antibody-loaded targeted ultrasonic microbubbles: An in vivo study in miniswine. OPEN LIFE SCI. 2023 Jan;18(1)Read more>>
  • Mingze Lu. et al. Camouflaged Nanoreactors Mediated Radiotherapy-Adjuvant Chemodynamic Synergistic Therapy. ACS NANO. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Qian-Qian Li. et al. Hollow Mesoporous Prussian Blue Nanoparticles Loaded with Daunorubicin for Acute Myeloid Leukemia Treatment. ACS APPL NANO MATER. 2023;XXXX(XXX):XXX-XXXRead more>>
  • Rintanalert Duangtawan. et al. SDF-1 involvement in orthodontic tooth movement after tooth extraction. SCI REP-UK. 2024 Feb;14(1):1-15Read more>>
  • Gong Wenchen. et al. Tertiary lymphoid structures as a potential prognostic biomarker for combined hepatocellularcholangiocarcinoma. HEPATOL INT. 2024 May;:1-16Read more>>
VALIDATION IMAGES