bs-4941R [Primary Antibody]
CD274 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CD274

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 8324

Source: KLH conjugated synthetic peptide derived from human CD274

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Programmed cell death 1 ligand 1(Programmed death ligand 1; PD-L1; PDCD1 ligand 1; B7 homolog 1; B7-H1; CD274) involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibit T-cell proliferation and cytokine production.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)

Predicted Molecular Weight: 32


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Huang Xianghua. et al. Expression of PD-L1 and BRCA1 in Triple-Negative Breast Cancer Patients and Relationship with Clinicopathological Characteristics. Evid-Based Compl Alt. 2021;2021:5314016Read more>>
  • Weiqi Meng. et al. ZhenQi FuZheng formula inhibits the growth of colorectal tumors by modulating intestinal microflora-mediated immune function. AGING-US. 2022 Jun 15; 14(11): 47694785Read more>>
  • Sun, Xinxin. et al. 0.1% Nano-silver mediates PD-1/PD-L1 pathway and alleviates chronic apical periodontitis in rats. ODONTOLOGY. 2022 Sep;:1-11Read more>>
VALIDATION IMAGES

Lane 1: Mouse Heart lysates; Lane 2: Mouse Skeletal muscle lysates; Lane 3: Rat Heart cell lysates probed with CD274 Polyclonal Antibody, Unconjugated (bs-4941R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.