bs-4986R [Primary Antibody]
rhIL-18 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: rhIL-18

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3606

Source: KLH conjugated synthetic peptide derived from human recombinant IL-18-Fc

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)

Predicted Molecular Weight: 22


Cross Reactive Species: Human

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xiuping Duan. et al. Metadherin silencing results in the inhibition of pyroptosis in lipopolysaccharide/adenosine triphosphate ? stimulated renal tubular epithelial cells. Tissue Cell. 2022 Apr;75:101722Read more>>
  • Jianzhao Liao. et al. Endoplasmic Reticulum Stress Contributes to Copper-Induced Pyroptosis via Regulating the IRE1_-XBP1 Pathway in Pig Jejunal Epithelial Cells. J Agr Food Chem. 2022;70(4):12931303Read more>>
  • Xuliang Zhang. et al. PINK1/Parkin-mediated mitophagy mitigates T-2 toxin-induced nephrotoxicity. FOOD CHEM TOXICOL. 2022 Jun;164:113078Read more>>
  • Jia, Ruxue. et al. Pyroptosis participates in PM2.5-induced airblood barrier dysfunction. ENVIRON SCI POLLUT R. 2022 Apr;:1-11Read more>>
  • Qiangbin Zhu. et al. Expression of histone H1 in rats with traumatic brain injury and the effect of the NLRP3 inflammasome pathway. WORLD NEUROSURG. 2022 DecRead more>>
  • Lu Yang. et al. Berberine attenuates depression-like behavior by modulating the hippocampal NLRP3 ubiquitination signaling pathway through Trim65. INT IMMUNOPHARMACOL. 2023 Oct;123:110808Read more>>
  • Huimin Yuan. et al. NLRP3 neuroinflammatory intervention of Mahuang-Lianqiao-Chixiaodou decoction for mental disorders in atopic dermatitis mice. J ETHNOPHARMACOL. 2023 Sep;:117263Read more>>
VALIDATION IMAGES

Lane 1: Human DU145 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human U-2OS cell lysates; Lane 4: Human A431 cell lysates; Lane 5: Human Siha cell lysates probed with rhIL-18 Polyclonal Antibody, Unconjugated (bs-4986R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.