bs-4995R [Primary Antibody]
Insulin receptor subunit beta Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Insulin receptor subunit beta

Immunogen Range: 801-900/1382


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3643

Swiss Prot: P06213

Source: KLH conjugated synthetic peptide derived from human Insulin receptor subunit beta

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)

Predicted Molecular Weight: 68/152


Cross Reactive Species: Human
Mouse
Rat
Others

Predicted Cross Reactive Species: Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Martinez-Rachadell L et al. Cell-specific expression of insulin/insulin-like growth factor-I receptor hybrids in the mouse brain. Growth Horm. IGF Res. 2019 Feb 22;45Read more>>
VALIDATION IMAGES

HEK 293T cells probed with Insulin receptor subunit beta Antibody, Unconjugated (bs-4995R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Formalin-fixed and paraffin embedded mouse lung labeled with Anti-Insulin receptor subunit beta/INSR/CD220 Polyclonal Antibody, Unconjugated (bs-4995R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded human kidney carcinoma labeled with Anti-Insulin receptor subunit beta/INSR/CD220 Polyclonal Antibody, Unconjugated (bs-4995R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Antigen: bs-4995P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;\nTMB staining;\nRead the data in MicroplateReader by 450