Host: Rabbit
Target Protein: SOAT2
Immunogen Range: 331-430/522
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 8435
Swiss Prot: O75908
Source: KLH conjugated synthetic peptide derived from human SOAT2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 60
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Pig
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
- Hung-Yu Sun. et al. Sterol O -acyltransferase 2 chaperoned by apolipoprotein J facilitates hepatic lipid accumulation following viral and nutrient stresses. Commun Biol. 2021 May;4(1):1-14Read more>>
- Shuzhou Wang. et al. Effects of NAC assisted insulin on cholesterol metabolism disorders in canine type 1 diabetes mellitus. LIFE SCI. 2022 Dec;:121193Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti SOAT2/ACAT2 Polyclonal Antibody, Unconjugated (bs-5020R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin-embedded human colon labeled with Anti-SOAT2/ACAT2 Polyclonal Antibody, Unconjugated(bs-5020R) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
L1 rat intestine lysates L2 rat brain lysates probed with Anti SOAT2/ACAT2 Polyclonal Antibody, Unconjugated (bs-5020R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at RT. Predicted band 57kD. Observed band size:57kD.
Lane 1: Mouse Liver tissue lysates; Lane 2: Rat Liver tissue lysates; Lane 3: Human HeLa cell lysates; Lane 4: Human Jurkat cell lysates; Lane 5: Human K562 cell lysates probed with SOAT2 Polyclonal Antibody, Unconjugated (bs-5020R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded (Human liver cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOAT2) Polyclonal Antibody, Unconjugated (bs-5020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOAT2) Polyclonal Antibody, Unconjugated (bs-5020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human duodenum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOAT2) Polyclonal Antibody, Unconjugated (bs-5020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human lung cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (SOAT2) Polyclonal Antibody, Unconjugated (bs-5020R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.